摘要
【目的】探究檀香紫檀植株高效、稳定的离体再生技术,为其苗木的规模化生产提供参考依据。【方法】以檀香紫檀种子为外植体,以MS培养基为基本培养基,添加不同浓度琼脂、蔗糖、IBA、IAA和6-BA进行无菌播种、诱导萌芽、增殖和生根培养,建立檀香紫檀组培快繁体系。【结果】檀香紫檀种子以0.1%HgCl2溶液消毒8 min的萌发率最高,为31.7%;微茎段芽萌发、生长及分化效果随培养基中IBA浓度提高而增强,当IBA浓度为0.3 mg/L时微茎段腋芽诱导率最高,为94.5%,且生长状况最佳,但与添加0.2 mg/L IBA的处理无显著差异(P>0.05);IBA促进微茎段愈伤组织发育和增殖的作用强于6-BA,二者配合使用的协同作用更明显,以培养基添加0.3 mg/L IBA和3.0 mg/L 6-BA对愈伤组织发育和芽梢增殖的效果最佳,增殖系数为2.8;接种在含1.0 mg/L IBA、0.25 mg/L IAA、0.05 mg/L 6-BA的生根培养基,微茎段生根率最高,为65.2%,且苗木生长发育最佳。【结论】以种子为外植体,通过无菌播种、微茎段增殖和生根培养可获得大量优质组培苗,是实现檀香紫檀工厂化育苗的理想途径。
【Objective】Exploring the efficient and stable regeneration technology of plantlets in vitro of red sandalwood(Pterocarpus santalinus)to provide reference for the large-scale production of the seedlings.【Method】Red sandalwood seeds were used as explants,MS medium was used as the basic medium,and different concentrations of agar,sucrose,IBA,IAA and 6-BA were added for aseptic sowing,germination,proliferation and rooting culture,a tissue culture rapid propagation system of red sandalwood was established.【Result】The seed germination rate was the highest after disinfection in 0.1% HgCl2 solution for 8 min,and the induction rate was 31.7%;the bud germination,growth and differentiation of sterile micro-stem increased with the increase of IBA concentration of the medium. When the concentration of IBA was 0.3 mg/L,the axillary bud induction rate of micro-stem was the highest(94.5%),and the growth status was the best,however,it was not significantly different from the medium of adding 0.2 mg/L IBA(P>0.05). The effect of 6-BA on the development and proliferation of micro-stem callus was stronger than that of 6-BA,but the synergy effect of the two was more effective,the callus growth and proliferation effect was the best when added 0.3 mg/L IBA and 3.0 mg/L 6-BA,and the proliferation coefficient was 2.8. When inoculated in rooting medium containing 1.0 mg/L IBA,0.25 mg/L IAA and 0.05 mg/L 6-BA,the rooting rate of the micro-stem segment was the highest,which was 65.2%,and the young plant growth and development was the best.【Conclusion】With seeds as explants,large-scale tissue culture seedlings can be obtained through sterile sowing,micro-stem proliferation and rooting culture. This is an ideal way to achieve industrial culture of seedlings of red sandalwood.
作者
徐呈祥
马艳萍
彭碧琳
潘建芳
廖苑蓓
XU Cheng-xian;MAYan-ping;PENG Bi-lin;PAN Jian-fang;LIAO Yuan-bei(College of Life Sciences,Zhaoqing University,Zhaoqing,Guangdong 526061,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2019年第12期2741-2748,共8页
Journal of Southern Agriculture
基金
国家自然科学基金项目(31270674)
广东省肇庆市科技计划项目(2019N012)
关键词
红木树种
檀香紫檀
无菌播种
组培繁殖
rosewood species
red sandalwood
aseptic sowing
propagation by tissue culture
