摘要
目的 评价异丙酚对人肺癌A549细胞凋亡及侵袭力的影响.方法 用RPMI-1640培养液将A549细胞株按2× 105个/ml细胞密度接种于96孔培养板和6孔培养板中,每孔分别为100μL和2 000μL,于5％ CO2、饱和湿度37℃培养24h.采用随机数字表法,将其分为2组（n=60）：二甲基亚砜组（DMSO组）和异丙酚组（P组）.P组加入异丙酚终浓度100 μmol/L; DMSO组加入终浓度0.5％DMSO.于药物孵育24h时,采用HCS法检测caspase-3的表达水平,Western blot法检测基质金属蛋白酶（MMP）-2的表达水平,于药物孵育0.5、1、5h时,采用Western blot法检测细胞外蛋白调节激酶（ERK）1/2的表达水平.结果 与DMSO组比较,P组caspase-3表达上调,MMP-2表达下调（P＜0.01）,药物孵育0.5h时ERK1/2表达上调,1h时其表达下调（P＜0.01）,5h时差异无统计学意义（P＞0.05）.结论 异丙酚可促进人肺癌A549细胞凋亡,抑制A549细胞的侵袭力.
Objective To evaluate the effects of propofol on apoptosis and invasiveness of human lung cancer cell line A549 cells.Methods Human lung cancer cell line A549 were seeded onto 96-well plates （100 μl/well） and 6-well plates （2 000 μl/well） at a density of 2× 105 cells/ml,and cultured for24 h at 37 ℃ in 5% CO2.The cells were randomly divided into 2 groups （n =60 each） using a random number table：dimethyl sulfoxide （DMSO） group and propofol group （group P）.In group P,propofol with the final concentration of 100 μmoYL was added.In group DMSO,0.5% DMSO with the final concentration of 0.5% was added.At 24 h of incubation with drugs,caspase-3 expression was detected by high content screening （HCS）; the expression of matrix metalloproteinase （MMP-2） was detected by Western blot analysis.At 0.5,1 and 5 h of incubation,ERK1/2 expression was also measured using Western blot analysis.Results Compared with group DMSO,the expression of caspase-3 was up-regulated,the expression of MMP-2 was down-regulated,ERK1/2 expression was up-regulated at 0.5 of incubation and down-regulated at 1 h of incubation,and no significant change was found in ERK1/2 expression at 5 h of incubation in group P.Conclusion Propofol can promote apoptosis in A549 cells and inhibit invasiveness of human lung cancer cell line A549 cells.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2014年第9期1079-1081,共3页
Chinese Journal of Anesthesiology
基金
浙江省卫生厅课题基金资助项目（2012RCA045）
