摘要
目的 初步建立长爪沙鼠自发型高脂血症模型的血清脂蛋白琼脂糖电泳检测方法和肠菌PCR-DGGE检测方法,用于今后评价沙鼠高脂血症模型.方法 选取444只长爪沙鼠,按性别年龄分组后CO2麻醉,取血液及肝脏标本,用于血清转氨酶、总胆固醇（TC）、甘油三脂（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）等指标的检测以及肝脏病理学的观察,血清脂蛋白电泳（琼脂糖电泳）进行高脂分类;取小肠、盲肠内容物用PCR-DGGE技术进行肠道微生物多样性检查,同时参照人类的高脂血症分类标准,对高龄组（平均12.81月龄）个体进行了高脂血症的分型.结果 约有10％～30％高龄组沙鼠其血清丙氨酸氨基转移酶（ALT）、门冬氨酸氨基转移酶（AST）、TG、TC、血糖（GLU）等指标明显高于低龄组（平均4.72月龄）沙鼠.病理学检查发现,高龄组肝脏出现较为明显的脂肪变性,而低龄组鼠肝脏无异常发现.DGGE结果表明,高龄组与低龄组沙鼠呈现出不同的带谱,后者条带数明显多于前者.高龄鼠从血脂指标上可分为五种类型,以对应于人的Ⅱ a、Ⅱ b、ⅢⅡ、Ⅳ、Ⅴ.结论 高沙鼠的自发性高脂血症或可以作为一个潜在的模型研究,琼脂糖电泳法和PCR-DGGE法或可作为评价高脂血症沙鼠模型的补充方法.
Objective To initially establish electrophoresis detection method of spontaneous hyper- lipidemia Mongolian Gerbil for the further evaluation of hyperlipidemia model, Methods The gerbils were divided into 7 groups, and anesthetized by CO2, then blood was collected for the detection of serum transaminases, CHO （TC）, TG, HDL, LDL and the liver tissues was collected for pathological observation. Serum lipoprotein electrophoresis （agarose gel electrophoresis） was conducted by SEBIA system.The ileum and caecum intestinal contents were collected and detected for the diversity of intestinal microor- ganisms by PCR-DGGE method. Referring to the classification criteria of human hyperlipidemia, the gerbil hyperlipidemia were typed. Results Approximatelyl0%-30% of older gerbils （averaged 12.81 months old） were spontaneous hyperlipidemia, their serum alanine aminotransferase, aspartate aminotransferase, triglycerides, total cholesterol, blood glucose and other indexes were significantly higher than youth groups （averaged 4.72 months old） （P〈0.05）. Pathological examination revealed that old group had more obvious hepatic steatosis. DGGE results showed that the model group and the young group presented different band spectrum. The band number of young group were significantly more than old group. Hyperlipidemia gerbils could be divided into five types according to the serum lipids indicators and 13 bands, corresponded to person＇s Ⅱa, Ⅱb, Ⅲ, Ⅳ, Ⅴ. Conclusions Spontaneous hyperlipidemia of old gerbils could be as a potential model for study, agarose electrophoresis and PCR-DGGE maybe as additional methods for evaluating hyperlipidemia gerbil models in the future.
出处
《实验动物与比较医学》
CAS
2014年第5期365-371,共7页
Laboratory Animal and Comparative Medicine
基金
浙江省自然科学基金（Y3080126）,浙江省公益类科技计划项目（2011C37096）,浙江省医药卫生平台研究计划（2011ZDA002）,省医药卫生优秀青年人才基金（2007QN001）,浙江省公共科技服务计划项目（2012F30026）
关键词
自发高脂血症
长爪沙鼠模型
PCR-DGGE
血清脂蛋白琼脂糖电泳
Spontaneous hyperlipidemia
Mongolian Gerbil model
PCR-Denaturing Gradient gel Electrophoresis （PCR-DGGE）
Serum lipoprotein electrophoresis
