摘要
建立HPLC-ELSD法测定冰酒中糖类含量的方法,应用高效液相色谱仪,NH2柱（5μm,4.6mm×250mm）,柱温40℃,流动相：乙腈∶水=80∶20,流速：0.8m L/min,蒸发光散射检测器检测,检测器温度为40℃,20μL进样。测得六种冰酒中主要糖类为果糖和葡萄糖,果糖含量大于葡萄糖含量,且各品种中的果糖和葡萄糖含量差别相对较大,6次平行进样,相对标准偏差为0.7%~2.1%,葡萄糖和果糖的加标回收率94.7%~103.4%,该方法具有准确,快速,操作简单等特点,可用于冰酒中糖类含量的检测。
A method was established to determine sugars in wine by using HPLC （High Performance Liquid Chromatography）with ELSD（evaporative light-scattering detector ） .The samples were separated on a NH2 （5μm 4.6mm × 25cm） chromatographic coLumn.The flowing phase was C2H3N ： H2O = 80 ： 20 and the velocity was 0.8mL/min. The temperature in column and detector was 40℃ and the input volume was 20μL.Experimental result showed that there were almost two types of sugar in six types of ice wine：fructose and glucose.Fructose content was more than glucose levels, and fructose and glucose content in each type of ice wine were difference .The relative standard deviation of this method was 0.7%-2.1 % （ n = 6）and the recovery was 94.7%- 103.6%.The method was accurate and simple,and was suitable for rapid detection glucose and fructose in ice wine.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第21期320-323,共4页
Science and Technology of Food Industry
