摘要
目的：建立三黄中空栓中黄芩苷和盐酸小檗碱的含量测定方法。方法：采用高效液相色谱法,色谱柱为Waters Symmetry Shield-C18柱（4.6 mm×250mm,5μm）,以0.1%磷酸水溶液-乙腈（50：50）为流动相,流速为1.0mL/min,检测波长265 nm,柱温为30℃进行检测。结果：黄芩苷线性范围为5.0~101.0μg/mL,平均加样回收率为98.80%（RSD%=1.17%）;盐酸小檗碱线性范围为5.7~114.0μg/mL,平均加样回收率为99.00%（RSD%=1.15%）。结论：该法快速、简便,结果准确、可靠,可用于三黄中空栓中黄芩苷和盐酸小檗碱含量的测定。
Objective： To establish content determination methods of baicalin and berberine hydrochloride contained in Sanhuang hollow suppository. Methods： HPLC was adopted, chromatographic column： Waters Symmetry Shield-C18 column （4.6 min×250 mm, 5μm）, 0.1% phosphoric acid solution-acetonitrile （50：50） were taken as the mobile phase, flow rate was 1.0 mL/min, detection wavelength was 265 nm, column temperature： 30℃. Results： Baicalin showed better linear relationship between 5.0 and 101.0μg/mL, average recovery rate was 98.80% （RSD= 1.17%）; berberine hydrochloride showed better linear relationship between 5.7 and 114.0μg/mL, average recovery rate was 99.00% （RSD=l. 15%）. Conclusion： The method, rapid, simple, accurate and reliable, could be used for content determination of baicalin and berberine hydrochloride contained in Sanhuang hollow suppository.
出处
《西部中医药》
2014年第9期19-21,共3页
Western Journal of Traditional Chinese Medicine
基金
甘肃省自然科学基金计划项目(编号2012-258)
