摘要
目的 观察重组人骨形态发生蛋白-2（rhBMP-2）复合β-磷酸三钙（β-TCP）及无机牛骨（Bio-Oss）对骨缺损修复能力的影响.方法 拔除10条Beagle犬下颌前磨牙,3个月骨质愈合后预备种植窝并制备5 mm×4 mm×3 mm骨缺损区,植入种植体,并分别在骨缺损区填入β-TCP、rhBMP-2/β-TCP复合物、Bio-Oss或rhBMP-2/Bio-Oss复合物;空白对照组不充填.术后4、12周处死犬,行大体观察、X线观察和组织形态学分析,应用图像分析软件分析缺损区骨密度改变及新生骨生成率.结果 植入骨替代材料的各实验组均有较好的新生骨.4周后,β-TCP/BMP及Bio-Oss/BMP组缺损区骨密度分别为（36.70±1.73）％和（38.50±1.83）％,高于原材料组[（30.50±1.81）％和（31.48±1.86）％.12周后,β-TCP/BMP及Bio-Oss/BMP组骨密度分别为（52.84±1.85）％和（54.85±1.87）％,也高于原材料组[（43.65±1.81）％和（44.94±1.88）％],且复合rhBMP-2的实验组新骨生成率更高.结论 rhBMP-2与人工骨替代材料复合后能够有效促进种植体周骨缺损的修复.
Objective To evaluate the bone repair capacity of β-tricalciumphosphate （β-TCP） or Bio-Oss combined with recombinant human bone morphogenetic protein-2 （rhBMP-2） in Beagle dogs.Methods Ten healthy adult Beagle dogs were used in this study.The mandibular premolars of the dogs were extracted firstly.Three months later,dental implants were placed in the mandibular,and a 5 mm× 4 mm× 3 mm bone defect area was prepared on the buccal side of each implant.The bone defect areas were filled with β-TCP,rhBMP-2/β-TCP compound,Bio-Oss or rhBMP-2/Bio-Oss compound respectively.The bone defect areas were filled nothing as the blank.The dogs were sacrificed after 4 or 12 weeks.Samples were prepared for the gross observation,X-ray analysis and histomorphology analysis.Results The new bone formation was observed in all experiment groups.After 4 weeks,the bone density of materials combined with rhBMP-2 groups [（36.70 ± 1.73） % and （38.50 ± 1.83） %] were higher than the raw materials groups [（30.50 ± 1.81）% and （31.48 ± 1.86）%].After 12 weeks,the bone density of materials combined with rhBMP-2 groups [（52.84 ± 1.85） % and （54.85 ± 1.87） %] were also higher than the raw materials groups [（43.65 ± 1.81） % and （44.94 ± 1.88） %].And new bone formation rate of the materials combined with rhBMP-2 groups were higher than the raw materials groups at each evaluation time.Conclusion rhBMP-2 can promote the bone regeneration activity of the artificial bone substitute materials effectively.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第10期2272-2274,共3页
Chinese Journal of Experimental Surgery
关键词
骨形态发生蛋白
人工骨替代材料
骨缺损
Bone morphogenetic protein
Artificial bone substitute materials
Bone defect
