摘要
以发芽黑米为原料,研究发芽黑米γ-氨基丁酸的提取工艺及其常压耐缺氧能力。研究结果表明:发芽黑米γ-氨基丁酸提取最佳工艺条件为超声提取时间为20.1 min,超声功率为239.6 W,液料比为19.0︰1(mL/g),乙醇体积分数为65.9%。测定常压缺氧条件下小鼠的惊厥时间和存活时间,总耗氧量和存活率,以及血浆促肾上腺皮质激素(ACTH)浓度和血清游离甲状腺素(FT4)浓度,表明发芽黑米γ-氨基丁酸具有较好的常压耐缺氧能力。发芽黑米γ-氨基丁酸能延长常压缺氧条件下小鼠的惊厥时间和存活时间,降低耗氧量和提高小鼠的存活率,降低ACTH浓度和提高FT4浓度。
Mainly discuss the extraction technology and anti-anoxia ability of γ-aminobutyric acid from black rice germination. The optimum extraction conditions of γ-aminobutyric acid from black rice germination were determined, such as ultrasonic extraction time for 20.1 min, extraction power for 239.6 W, the solution and material ratio for 19.0︰1(mL/g), and ethanol concentration for 65.9%. The atmospheric convulsion time and survival time, total oxygen consumption and the survival rate, plasma adrenocorticotropic hormone concentration and Serum free thyroxine concentration of mice was tested. It shows that γ-aminobutyric acid from black rice germination can prolong atmospheric convulsion time and survival time, decrease oxygen consumption and increase the survival rate of mice, and decrease the concentration of plasma adrenocorticotropic hormone and increased the concentration of Serum free thyroxine of mice as well. Thus, γ-aminobutyric acid from black rice germination has a good anti-anoxia ability.
出处
《食品工业》
北大核心
2014年第10期49-53,共5页
The Food Industry
关键词
发芽黑米
Γ-氨基丁酸
提取
耐缺氧
black rice germination
γ-aminobutyric acid
extraction
anti-anoxia