摘要
目的采用超高效液相色谱与串联四级杆飞行时间质谱仪联用技术(UPLC-Q-TOF-MS/MS)分析鉴定大鼠灌胃马钱苷后其在尿液中的代谢产物,阐明马钱苷代谢途径。方法大鼠灌胃给予马钱苷,收集给药前后的尿样,经固相萃取进行预处理,采用超高液相色谱C18色谱柱,流动相为乙腈-1%甲酸水,梯度洗脱,采用动态背景扣除(DBS)触发信息关联采集(IDA)进行MS/MS数据采集。结果经MetabolitePilot代谢物分析软件处理后,根据MS/MS给出质谱碎片信息对代谢产物进行结构推测,共鉴定马钱苷代谢物3种,并归纳了马钱苷在大鼠体内的代谢过程。结论该方法简单可靠,灵敏度高,可用于体内微量成分的分析鉴定。
Objective To identify the metabolites of loganin in rat urine through using ultra high performance liquid chromatography combined with triple TOF mass spectrometry （UPLC-Q-TOF/MS/MS） after oral administration of loganin;To summarize the metabolic pathway of loganin. Methods Rats were given a gavage with loganin. The urine samples were prepared by use of solid phase extraction （SPE）. The chromatographic separation was performed on an UPLC C18 column with gradient elution program of acetonitrile and 0.1% formic acid aqueous as mobile phase. Dynamic background subtraction （DBS） technology was employed to trigger information dependent acquisition （IDA） to obtain the characteristic fragment ions of metabolites. Results MetabolitePilot software including several data processing methods were employed to speculate the structures of liganin metabolites and three metabolites were identified. Then, the metabolic pathway of loganin in rats was summarized. Conclusion The analytical method was simple, credible and highly-sensible, which is useful for screening and identifying trace constituents in vivo.
出处
《中国中医药信息杂志》
CAS
CSCD
2014年第10期77-79,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家科技重大专项-重大新药创制(2011ZX09201-201-28)
