摘要
目的通过对四妙勇安汤水提物入血成分的研究,初步阐明该复方的药效物质基础。方法采用高效液相色谱-离子阱-飞行时间质谱(LCMS-IT-TOF)联用技术,在电喷雾离子源的正、负离子模式下,对四妙勇安汤水提物、大鼠灌胃给药后的血清和空白血清进行检测。以甲醇对血清样本进行前处理,采用Inertsil ODS-3色谱柱,以乙腈(A)-水(含0.1%甲酸)(B)为流动相进行梯度洗脱,根据化合物的多级质谱碎片,结合参考文献和对照品初步确定四妙勇安汤水提物入血成分。结果四妙勇安汤灌胃给药后在大鼠血清中发现14个入血成分,其中10个来自该复方的原型成分,其余4个可能为代谢产物。9个色谱峰被确认为哈巴苷、绿原酸、马钱苷、当药苷、甘草苷、金丝桃苷、安格洛苷C、异绿原酸C和哈巴俄苷。结论四妙勇安汤入血成分是各组方药味配伍后共同作用的结果,本研究可为四妙勇安汤的药效物质基础和进一步的体内代谢研究提供参考。
Objective To study the absorbed constituents of water extract of Simiao Yong＇an Decoction（ SYD） in serum of rats,so as to find out the pharmacodynamics material basis. Methods Using liquid chromatograph-mass spectrometry（ LCMS）-ion trap（ IT）-time of flight（ TOF） method under both positive and negative ion modes of electrospray ionization,the water extract of SYD,rats＇ serum after oral administration of SYD and the blank serum were analyzed. Serum samples were pretreated by using methanol. The chromatographic condition consisted of Inertsil ODS-3 column,mobile phase of acetonitrile（ A） and water（ 0. 1% formic acid contained）（ B） with gradient elution. The compounds absorbed in serum was identified by multiple stage mass spectrum fragments,published reports and standard substances. Results Finally,14 compounds were preliminary identified in rats serum,of which ten compounds were prototypes of SYD and four were metabolites. Nine peaks were validated as harpagide,chlorogenic acid,loganin,sweroside,liquiritin,hyperoside,angoroside C,isochlorogenic acid C and harpagoside. Conclusion Constituents of SYD in serum were generated from compatibility of all herbal medicines. This study would contribute to the further study of pharmacodynamics properties and metabolism of SYD.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2016年第11期933-940,共8页
Journal of Beijing University of Traditional Chinese Medicine
基金
北京中医药大学自主选题项目(No.2016-JYB-XS050)
关键词
四妙勇安汤
入血成分
高效液相色谱-离子阱-飞行时间质谱
代谢产物
Simiao Yong ＇an Decoction
constituents absorbed in serum
liquid chromatograph-mass spectrometry-ion trap-time of flight
metabolites
