摘要
目的建立离子色谱法测定炎琥宁原料药中碳酸盐残留的方法。方法采用离子色谱法。色谱柱：Dionex IonPac AS18（250 mm×4 mm）;淋洗液：0.022 mol/L氢氧化钾;体积流量：1.0 mL/min;进样体积：10μL;柱温：30℃;检测器：电导检测器;检测池温度：35℃;抑制器：Dionex ASRS 300（4 mm）。结果 CO32-与Cl-、NO2-、NO3-、SO42-分离良好,CO32-在10~120μg/mL与峰面积呈良好的线性关系（r=0.999 0）;平均回收率为102.1%,RSD值为3.7%（n=9）。结论该方法灵敏、准确、简便,专属性好,可用于测定炎琥宁原料药中碳酸盐的残留。
Objective To establish a method to determine the residual of carbonate in potassium sodium dehydroandroan drographolide succinate by ion chromatography. Ion chromatography was used. The separation was carried out on a Dionex IonPac AS18 column （250 mm × 4 mm） with 0.022 mol/L KOH as mobile phase. The injection volume was 10 μL at the flow rate of 1.0 mL/min. The temperature of column was set at 30 ℃. Conductivity detector with temperature of detector cell 35 ℃ was used. Dionex ASRS 300 （4 mm） was used as suppressor. Results There was good separation between CO32-and CI-, NO2-, NO3-, and SO42-. The standard curve was rectilinear in the range of 10 -- 120 μg/mL （r=0.9990）. The average recovery was 102.1% with RSD value of 3.7% （n = 9）. Conclusion This method is accurate, simple, and selective which could be used for the determination of the residual of carbonate in potassium sodium dehydroandroan drographolide succinate.
出处
《现代药物与临床》
CAS
2014年第9期992-994,共3页
Drugs & Clinic
关键词
炎琥宁
碳酸盐
离子色谱
potassium sodium dehydroandroandrographolide succinate
carbonate
ion chromatography
