摘要
目的:建立离子色谱法测定口服补液盐散(Ⅲ)中总氯的含量。方法:采用IonPac AS 14A色谱柱(250 mm×4mm)和IonPac AG 14A保护柱(50 mm×4 mm),以9 mmol·L^(-1)的碳酸钠溶液为淋洗液,等度洗脱,流速为1.0 ml·min^(-1),进样量25μl;AERS 500(4 mm)抑制器,抑制电流:45 mA;以电导检测器(ECD)检测,氯离子(Cl^-)的含量按峰面积以外标法计算。结果:氯离子(Cl^-)的线性范围为0.05~20.00 mg·L^(-1)(r=0.999 9);回收率为98.89%(RSD=0.56%,n=6)。结论:本方法灵敏度高,前处理简单,准确可靠,可用于口服补液盐散(Ⅲ)中总氯的含量测定。
Objective： To establish a method for the determination of total chloride in oral rehydration salts powder （ Ⅲ ） by ion chromatography （IC）. Methods： The analysis was performed on an IonPac AS 14A （250 mm x4 ram） column with an IonPac AG 14A （50 mm ×4 mm） guard column and 9mmol·L^-1 Na2CO3solution as the mobile phase. The flow rate was 1.0 ml ·min^-1 with isocratic elution and the sample size was 25 μl. The suppressor was AERS 500（4mm） with 45mA suppression current, and an ECD was used for the detection. The quantity of chloride ion （ Cl^-1 ） was calculated by the peak area with an external standard method. Results ： The linear range of Ct - 1 was 0.05-20.00 mg ·L^-1（ r = 0. 999 9 ） ; the recovery was 98.89% （ RSD = 0.56%, n = 6 ）. Conclusion： The results demonstrated that the method has the advantages of high sensitivity, simple sample pretreatment, promising accuracy and good reliability, which is suitable for the determination of total chloride in oral rehydration salt powder （ Ⅲ）.
作者
胡建勇
罗廷顺
张丽华
Hu Jianyong Luo Tingshun Zhang Lihua(Dali Institute for Food and Drug Control, Yunnan Dali 671000, China Dali Second People＇s Hospital)
出处
《中国药师》
CAS
2017年第9期1689-1691,共3页
China Pharmacist
关键词
离子色谱
口服补液盐散(Ⅲ)
总氯
Ion chromatography
Oral rehydration salt powder （ Ⅲ）
Total chloride
