摘要
目的:探讨复方茵柏颗粒对四氯化碳(CCl4)致肝损伤大鼠TNF-α及细胞凋亡的影响。方法:大鼠腹腔单次注射40%CCl4玉米油制备急性肝损伤模型,将大鼠随机分为6组:正常对照组、模型组、复方茵柏颗粒低、中、高剂量组(2.16 g·kg-1、4.32 g·kg-1、8.64 g·kg-1)、阳性对照组(复方茵柏合剂8.64 g·kg-1),用酶联免疫吸附法测定血清TNF-α水平,用TUNEL法检测组织凋亡细胞,用酶联免疫吸附法检测肝匀浆Caspase-3的活性。结果:复方茵柏方能明显降低肝损伤组大鼠血清TNF-α水平,抑制细胞凋亡,并明显降低肝匀浆Caspase-3的蛋白表达量,与模型组比较有显著差异(P<0.01,P<0.05)。结论:复方茵柏颗粒抗CCl4所致肝损伤的作用与抑制TNF-α分泌、抑制细胞凋亡有关。
Objective：To investigate the effects of complex prescription Yinbai Granule on TNF- α and hepatic cell apoptosis with acute liver injury induced by carbon tetrachloride in rats. Methods：Acute liver injury was induced by 40%carbon tetrachloride in rats. Rats were randomly divided into 6 groups： normal control group,model group,complex prescription Yinbai Granule low dosage,middle dosage and high dosage groups（2. 16 g·kg^-1,4. 32 g·kg^-1,8. 64 g·kg^-1）,positive control（complex prescription Yinbai mixture 8. 64 g·kg^-1）. Enzyme- linked immunosorbent assay（ELISA） was used to detect the TNF-α content in serum and Caspase-3 protein expression in liver homogenate. The apoptosis index（AI） of hepatic cell was measured by TUNEL method. Results：After being treated by complex prescription Yinbai Granule,the serum level of TNF- α and apoptosis index was decreased obviously in rat with liver injury induced by CCl4（P〈0.01,P〈0.05）. Caspase-3 protein expression in liver homogenate was decreased significantly（compared with liver injury group,P〈0. 01）. Conclusion：Complex prescription Yinbai Granule has protection effect on acute hepatic injury in rats induced by carbon tetrachloride and the mechanism is related to inhibiting TNF- α and cell apoptosis.
出处
《中华中医药学刊》
CAS
2014年第10期2434-2436,I0014,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科学研究基金计划项目(2012ZB133)
杭州市卫生科技计划项目(2012A036)
关键词
复方茵柏颗粒
急性肝损伤
TNF-Α
细胞凋亡
complex prescription Yinbai Granule
acute liver injury
TNF-α
cell apoptosis
