摘要
目的观察益气养阴活血法对糖尿病模型大鼠血清NF-kB的影响。方法取大鼠55只,分为5组,除正常对照组外,其余组给予高脂饲料,按30mg/kg给大鼠腹腔单次注射链脲佐菌素(STZ)溶液1次,造模成功后除模型组和正常对照组外,各组连续灌胃药物4周,取血测定血糖、NF-kB,监测尿白蛋白、肾重量。结果造模后NF-kB的表达明显升高,与正常组比较有十分显著性差异(P<0.01)。给予丹蛭降糖方灌胃后,NF-kB的高表达较模型组明显降低(P<0.01),尿微量白蛋白排泄显著下降(P<0.05),血糖与治疗前比较明显降低(P<0.01)。结论具有益气养阴活血作用的丹蛭降糖胶囊可控制NF-kB的高表达,减少尿白蛋白的排泄,降低血糖。
Objective The aim of this study was to investigate the effects of supplementing qi,nourishing yin and remove blood stasis method on the expression of NF-kB in type 2 diabetes rodent model. Methods 55 Wistar rats were randomly assigned to 5 groups. All animals in the experimental group were fed a high calorie diet and were injected with streptozocin( STZ,30 mg /kg),except the control group.When diabetes rodent model was successfully builded,the animals in the experimental group were kept with intragastric administration for 4weeks. After that,blood glucose,NF-kB and urinary albumin levels were measured for all animals. Results The expression of NF-kB was significantly increased compared to the control group. However,the expression of NF-kB was significantly decreased in animals treating with DJC compared to the model group( P < 0. 01),and the excretion of urinary albumin declined significantly( P < 0. 05). The level of blood glucose also was significantly decreased,compared to the model group( P < 0. 01). Conclusion DJC controls the over-expression of NF-kB,decreases the excretion of urinary albumin and the level of blood glucose effectively.
出处
《湖北中医药大学学报》
2014年第3期9-11,共3页
Journal of Hubei University of Chinese Medicine
基金
国家中医管理局项目(国中医药科技发(2012)39号
JDZX2012004)
关键词
益气养阴活血法
糖尿病模型大鼠
核因子-KB
尿白蛋白
supplementing qi,nourishing yin and remove blood stasis method
diabetes rats
NF-kB
urinary albumin
