摘要
应用本实验室筛选得到的纤维素酶高产菌株里氏木霉(Trichoderma reesei)150-1-1发酵制备纤维素酶固体曲,通过优化固态发酵培养基组成及发酵条件,制备得到纤维素酶活力较高的固体曲及粗酶液。实验结果表明,在原料量为10 g,麸皮与秸杆粉质量比为4∶1,料液比为1∶2.5,发酵时间为120 h,发酵温度为31℃,发酵起始pH值为5.5的条件下,应用里氏木霉150-1-1发酵得到的纤维素酶固体曲酶活力达到423.6 U/g。
The cellulase solid koji was fermented by using high yielding strain Trichoderma reesei 150-1-1 screened in our laboratory. Solid koji and crude enzyme with high cellulase activities were obtained by optimizing the medium components and fermentation conditions of solid fermentation. The results showed that the cellulase activities of solid koji reached 423. 6 U/g under the optimal conditions where the fermentation material was 10 g,the ratio of straw powder to wheat bran was 4：1,the ratio of materials to water was 1：2. 5,the fermentation reaction temperature was 31℃,the reaction time was 120 h,and the original pH was 5. 5.
出处
《生物质化学工程》
CAS
2014年第5期7-10,共4页
Biomass Chemical Engineering
基金
国家林业局公益性行业专项资助项目(201004001)
江苏省自然科学基金资助项目(BK2012515)
关键词
里氏木霉
纤维素酶
固体曲
发酵
Trichoderma reesei
cellulase
solid koji
fermentation
