摘要
目的探讨雌激素对体重内稳态调节及水-甘油通道蛋白(AQPs)表达的影响。方法雌性SD大鼠32只随机分为4组,假手术组、去卵巢组,100、400μg/kg雌二醇组,连续给予受试物2周。定期观察体重和摄食量变化、血糖仪检测血糖、实时荧光定量PCR检测脂肪和肝脏组织水-甘油通道蛋白(AQPs)、沉默信息调节因子1(Sirt1)基因表达水平。结果去卵巢大鼠摄食量增加,平均体重[(330.44±25.09)g]明显高于假手术组[(287.44±13.18)g](P<0.05),脂肪系数(1.55±0.48)明显高于假手术组(0.93±0.19)(P<0.05),口服葡萄糖90 min后血糖浓度[(11.30±0.50)mmol/L]明显高于假手术组[(8.23±1.39)mmol/L](P<0.05),肝脏组织AQP9表达水平(1.37±0.17)明显高于假手术组(1.04±0.10)(P<0.05)。而给予去卵巢大鼠雌激素后,其摄食量和体重下降,子宫周围脂肪组织AQP7和肝脏组织AQP9表达水平明显降低。结论雌激素缺乏会导致大鼠体重上升,雌激素可能通过影响摄食行为、胰岛素敏感性、改变AQP表达等参与体重内稳态调节。
Objective To explore whether estrogen is involved in the regulation of weight homeostasis and impacts of estrogen on expression levels of aquaglyceroporin（AQP）.Methods Female Sprague-Dawley（SD） rats were randomly divided into four groups（n = 8 for each group）：a sham-operated group（SO）,and three ovariectomized groups（OVX）.Four weeks after the operation,the rats were administrated with corn oil（SO）,corn oil（OVX）,100 μg / kg 17β-estradiol（E2）（OVXE1） and 400 μg/kg E2（OVXE2） via subcutaneous injection for 2 weeks.The body weight and food consumption were monitored.The levels of glucose in blood were detected with glucometer.The mRNA expression levels of AQP and silent information reguloaor 1（Sirt 1） in parametrial adipose tissues and liver were determined with real-time PCR method.Results The OVX rats（330.44 ± 25.09 g） weighed heavier than SO rats（287.44 ± 13.18 g）,with a statistically significant difference（t = 3.71,P 〈0.05）.The organ coefficient of parametrial adipose tissues was greater in OVX rats（1.55 ± 0.48） than that in SO rats（0.93 ± 0.19）（t = 2.79,P 〈0.05）.The glucose level in OVX rats reached the maximum（11.30 ± 0.50 mmol /L） in 90 min after glucose gavage,which was significantly higher than that in SO rats（8.23 ± 1.39 mmol /L）（t = 3.60,P 〈0.05）.The AQP9 expression level in liver significantly increased in OVX rats（1.37 ±0.17） than that in SO rats（1.04 ±0.10）（t =3.86,P 〈0.05）.Administration of E2 to OVX rats significantly reduced the food consumption,body weight,and the expression levels of AQP7 in parametrial adipose tissues and AQP9 in liver.Conclusion Estrogen deficiency induces weight gain,while estrogen replacement could reverse it.Estrogen may participate in the regulation of weight homeostasis by influencing food intake,affecting insulin sensitivity,and modifying expression levels of AQP.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2014年第10期1276-1278,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(21347005
21277078)
江苏省自然科学基金(BK20131202
YKC13041)
