摘要
目的:观察三七总皂苷(panax notoginseng saponins,PNS)对兔骨髓间充质干细胞(bone marrow stromal cells,BMSCs)成骨分化及TGF-β1在基因水平表达变化的影响。方法:体外分离培养兔BMSCs,观察不同浓度PNS(50、100、200 mg/L)对兔BMSCs的影响,采用MTT法检测细胞增殖,茜素红染色观察钙结节形成,qRT-PCR检测TGF-β1表达水平。结果:各浓度PNS培养下的细胞增殖无明显差异(P>0.05);100、200 mg/L PNS组的钙结节数量和TGF-β1的表达水平均高于阴性组(P<0.05)。结论:PNS可促进兔BMSCs的成骨分化并刺激其分泌TGF-β1,但无明显促细胞增殖作用。
Objective： To observe the osteoinduction effect of panax notoginseng saponins （PNS） on rabbit bone marrow mesenchymal stem cells （BMMSCs）, and the expression level change of TGF-β1. Methods： Rabbit BMMSCs were isolated and cultured in vitro. To observe the influence of different concentration of PNS （50、100、200 mg/L） on rabbit BMMSCs,several methods were performed, including cell culture technique, MTT colorimetric assay, Dahl McGec Russell＇s Alizarin red staining for calcium and qRT-PCR explore TGF-β1 expression level. Results： There was no significant difference of proliferation capacity of BMMSCs between various concentration of PNS （P 〉0.05）. The calcium nodule and TGF-β1expression level in 100、200 mg/L PNS groups were significantly higher than negative group （P〈0.05）. Conclusion： PNS can promote the osteogenic differentiation and secretion of TGF-β1 of rabbit BMMSCs, but PNS is not effective enough for mediation of BMMSCs proliferation.
出处
《口腔颌面外科杂志》
CAS
2014年第4期261-265,共5页
Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金资助项目(81160132)
广西自然科学基金(0991013)
关键词
三七总皂苷
成骨分化
骨髓间充质干细胞
转化生长因子
panax notoginseng saponins
osteogenic differentiation
bone mesenchymal stem cells
transforming growth factor
