摘要
目的:探讨Wnt/β-catenin通路激活剂—氯化锂(lithium chloride,LiCl)对体外培养的人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)增殖和成骨分化的影响。方法:低密度多克隆法分离培养人PDLSCs,分别与不同浓度的LiCl(5、10、20、40 mmol/L)共同培养。分别检测各组细胞的增殖情况、碱性磷酸酶(alkaline phosphatase,ALP)活性、钙化结节形成量以及Col-1、OCN、Runx-2等成骨相关基因的表达水平。结果:LiCl无促细胞增殖作用,且高浓度LiCl能明显抑制细胞的增殖。浓度为5 mmol/L的LiCl可促进hP-DLSCs成骨性分化,表现为提高细胞的ALP活性、促进钙化结节的形成、上调Col-1、OCN、Runx-2的mRNA表达水平,与对照组相比,差异均具有统计学意义(P<0.05)。而高浓度(≥10 mmol/L)LiCl则对成骨分化具有抑制作用。结论:终浓度为5 mmol/L的LiCl可明显促进hPDLSCs的成骨分化。
AIM： To investigate the effects of lithium chloride （LiC1） , a Wnt/β-catenin pathway activator, on the proliferation and osteogenic differentiation of human periodontal ligament stem cells （hPDLSCs） in vitro. METHODS ： hPDLSCs were cultured as reported in the literature. The cells of passage 3 （P3） were cultured in normal medium （for cell proliferation assay） or osteogenic medium （for osteogenic differentiation assay） containing LiC1 at 5, 10, 20, and 40 mmoL/L, respectively, while the medium without LiC1 served as the control. The cell proliferation was assayed by MTT method, while the osteogenic differentiation markers including alkaline phosphatase （ALP） activity, the quantity of calcified nodules, and the gene expression of Col-1, OCN, and Runx-2 were evaluated and quantified. RESULTS ： LiC1 inbibited hPDLSCs proliferation in a dose and time - dependent manner. LiC1 at 5 mmol/L increased the ALP activity （ P 〈 0.05 ） and the amount of calcified nodules, enhanced the mRNA levels of Col- 1, OCN and Runx-2 in hPDLSCs（P 〈0.5 ） ; While LiC1 at 10 and 20 mmol/L decreased ALP activity, calcified nodules and mR- NA levels of Col-l, OCN and Runx-2 in the cells（P 〈 0.05）. CONCLUSION： LiC1 at 5 mmol/L can promote os- teogenic differentiation of hPDLSCs.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2013年第1期12-16,共5页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目(81071253
31170912
31030033)
国家重点基础研究发展计划项目(973计划)(2010CB944800)
关键词
氯化锂
牙周膜干细胞
增殖
分化
lithium chloride
periodontal ligament stem cells
proliferation
differentiation
