摘要
目的观察茶多酚对内毒素脂多糖(LPS)作用的人牙周膜细胞增殖和碱性磷酸酶活性的影响,为茶多酚在牙周疾病的防治中提供一定依据。方法体外培养人牙周膜细胞,采用MTT比色法观察茶多酚对LPS作用的人牙周膜细胞增殖活性的影响,通过碱性磷酸酶(ALP)活性测定法观察茶多酚对人牙周膜细胞的分化作用。结果100μg/ml LPS可抑制人牙周膜细胞的增殖活性和碱性磷酸酶活性。加入LPS同时分别给予50～1100μg/ml不同浓度茶多酚,能对抗LPS的抑制作用,增强人牙周膜细胞的增殖活性和碱性磷酸酶活性,其中在800μg/ml作用24h时促进作用达到最强。结论茶多酚可对抗内毒素对人牙周膜细胞的毒性作用,促进细胞的增殖和骨向分化。
Objective Observing the effects of tea polyphenol on the proliferation of human periodontal ligament cells （HPDLCs） affected by the lipopolysaccharide （LPS） and the activity of alkaline phosphatase （ALP） to provide certain evidence to prove that the tea polyphenol can be used to prevent and cure the periodontal disease. Methods Human periodontal ligament cells were primarily cultured in vitro; then the MTT colorimetric assay is adopted to observe the effect of tea polyphenbl on the proliferation of human periodontal ligament cells （HPDLCs） affected by the lipopolysaccharide （LPS） and ALP activity assay is also used to observe the effect of tea polyphenol on the differentiation of the human periodontal ligament cells affected by LPS. Results 100ug/ml LPS can be used to inhibit the proliferation activity and ALP activity of human periodontal ligament cells. While adding LPS, different concentration of tea polyphenols from 50ug/ml to 1100 ug/ml can be offered to withstand the inhibitory action of LPS and improve the proliferation activity of human periodontal ligament cells as well as the ALP activity, in which the utmost facilitation can be achieved at 800 ug/ml with an action time of 24 hours. Conclusion Tea polyphenols can be used to withstand the toxic effect of LPS on the human periodontal ligament cells and promote proliferation and osteogenic differentiation of cells.
出处
《现代口腔医学杂志》
CAS
CSCD
2012年第4期266-268,261,共4页
Journal of Modern Stomatology
基金
兰州大学学生创新创业行动计划项目(2011~514)
关键词
茶多酚
人牙周膜细胞
脂多糖
增殖
碱性磷酸酶
Tea polyphenols Human periodontal ligament cells LPS Proliferation Alkaline phosphatase
