摘要
采用拮抗试验和酯酶同工酶电泳技术对15个香菇菌株进行了菌种遗传特异性研究。结果表明：15个香菇菌株的谱带都在8-11条之间,酶谱稳定,可重复性高,酯酶同工酶技术可应用于香菇种群的划分研究;聚类分析结果显示,在相似水平为0.41时,15个供试菌株聚合为6类：第1类包括‘荷香1号’、‘浙江丽水808’和新宾县‘东升808’,其中引自浙江丽水的‘808’和新宾‘东升808’菌株相似系数为100%,说明其遗传背景相同,为同名同种;第2类包括丹东‘早丰8号’;第3类包括‘向阳2号’和‘辽抚4号’;第4类包括辽宁省农业科学院提供的‘937’、引自新宾东升的‘937’、‘抚香3号’、‘锦香’和‘永香1号’,其中辽宁省农业科学院提供的‘937’和引自新宾东升的‘937’菌株相似系数为100%,说明其遗传背景相同,为同名同种;第5类包括‘平泉18’、新宾二道‘早丰8号’和新宾‘永陵808-2’;第6类包括‘天成1号’,聚类分析结果与拮抗试验结果基本一致。
Genetic specificity of 15 Lentinula edodes strains were studied using antagonism experiment and the technology of esterase isozyme electrophoresis in this paper.The results showed that the quantity of bands of 15 Lentinulaedodes strains were in the 8-11range,enzyme spectrum stability,high repeatability,which showed that esterase isozyme technique could be applied to the taxonomy study of Lentinula edodes species;cluster analysis results showed that in the similar level of 0.41,15 strains aggregated into six types：category 1includes‘Hexiang 1＇,‘808＇from Lishui city of Zhejiang province and‘808＇from Dongsheng of Xinbin country among which the strains similarity coefficient of‘808＇from different place was 100%,testifying the same genetic background and with the same name;category 2includes‘Zaofeng 8＇from Dandong city;category 3includes‘Xiangyang 2＇and‘Liaofu 4＇;category 4includes‘937＇from Liaoning academy of agricultural sciences,‘937＇from Dongsheng of Xinbin country,‘Fuxiang 3＇,‘Jinxiang＇and‘Yongxiang 1＇,among which the strains similarity coefficient of‘937＇from different place was 100%,testifying the same genetic background and with the same name.category 5includes‘Pingquan 18＇,‘Zhaofeng 8＇from Erdao of Xinbin country and‘808-2＇from Yongling of Xinbin country;category 6includes‘Tiancheng 1＇.Cluster analysis results and the results of antagonism test were basically the same.
出处
《北方园艺》
CAS
北大核心
2014年第18期163-166,共4页
Northern Horticulture
关键词
香菇
拮抗试验
酯酶同工酶
聚类分析
Lentinula edodes
antagonism test
esterase isozyme
cluster analysis
