摘要
本研究利用不连续聚丙烯酰胺凝胶电泳法（ＰＡＧＥ）对绵羊的８种组织器官（心脏、肝、肺、脾、肾、骨骼肌、晶状体、血清）ＬＤＨ同工酶的分布及相对活性进行了分析测定，比较ＬＤＨ同工酶的酶谱特征。结果表明，绵羊的ＬＤＨ同工酶分布特征不同于其它动物，其相对活性有显著差异。骨骼肌中ＬＤＨ５活性比ＬＤＨ１强，Ｍ亚基比例大于Ｈ亚基；肝脏和心脏中ＬＤＨ１的活性最强。
The LDH isoenzyme distribution and its relative activity in heart,liver,lung,spleen,skeletal muscle,crystalline lens and serum of sheep were analysed using Polyacylamide Gel Electrophoresis and electropherogram scanning techniques to compare the characteristics of electropherogram of LDH isoenzymes in different tissues of sheep The results showed that the LDH isoenzyme distribution was different from that of other animals,and its relative activity demonstrated a significant difference;LDH 5 activity in skeletal muscle was much higher than that of LDH 1 with higher percentage of M subunit than that of H subunit The LDH 1 activity in heart and liver was the strongest
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1997年第5期401-403,共3页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
