摘要
根据GenBank登录的鹅细小病毒(GPV)和番鸭细小病毒(MDPV)非结构蛋白(NS)基因特征,本研究设计1对特异性引物对GPV和MDPV基因组DNA进行PCR扩增,目的片段大小均为810bp,并对PCR产物进行切胶回收。用EcoRⅠ酶对GPV和MDPV特异性胶回收产物进行酶切鉴定,结果显示MDPV经EcoRⅠ酶切后琼脂糖凝胶电泳检测片段为2段,大小为530和280bp;而GPV经EcoRⅠ酶切后琼脂糖凝胶电泳检测片段大小不变。本研究建立了一种快速区别GPV和MDPV感染的检测方法,可对番鸭感染水禽细小病毒的情况进行快速鉴别诊断。
A pair of specific primers was designed to amplify the goose parvovirus （GPV） and Muscovy duck parvovirus （MDPV） and establish a polymerase chain reaction and restriction fragment length polymorphism （PCR RFLP） method for rapid detection of the infection status of waterfowl. An EcoR I restriction enzyme was used which could digest MDPV PCR fragments into two different parts, with gene lengthes of 530 and 280 bp, while GPV PCR fragments couldn＇t be digested by EcoR I restriction enzyme. We established a fast differential diagnosis for GPV and MDPV using only one pair of primers and a restriction enzyme.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第9期56-59,共4页
China Animal Husbandry & Veterinary Medicine
基金
公益性行业(农业)科研专项经费(201003012)
