摘要
为制备鹅细小病毒(goose parvovirus,GPV)单克隆抗体(monoclonal antibody,McAb),利用浓缩的GPV免疫BALB/c小鼠,采用杂交瘤技术,经间接ELISA方法筛选和有限稀释法克隆后获得一株抗GPV单克隆抗体杂交瘤细胞株4B4。经鉴定,其McAb的重链为IgG1亚型,轻链为κ链。ELISA和Western blotting试验结果表明,获得的4B4株McAb可特异性的识别GPV和GPV-VP3蛋白,表明4B4株McAb识别的表位可能位于GPV VP3蛋白的保守区域。为进一步鉴定GPV的表位和GPV诊断试剂的研究奠定了基础。
In order to prepare the monoclonal antibody of goose parvovirus,BALB/c mice were immunized by concentrated GPV and anti-GPV hybridoma cells（4B4） were obtained by hybridoma technique,indirect ELISA and limiting dilution assay.After identification,the heavy chain of McAb was IgG1 subtype and the light chain was κ chain.The results of ELISA and Western blotting showed that GPV and GPV-VP3 protein could be specially recognized by 4B4 McAb.It indicated that the epitope recognized by 4B4 McAb might be the conservated region of GPV VP3 protein.This research settled the foundation for the characterization of GPV epitope and the investigation of GPV diagnostic reagent.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第2期162-164,共3页
China Animal Husbandry & Veterinary Medicine
基金
吉林省牧业管理局项目(吉牧科字第200902号)
