摘要
根据鹅细小病毒GPVH1株结构蛋白VP1与VP3编码基因非重叠核苷酸序列设计了一对引物GPR1 /GPR2 ,用这对引物对感染器官内的GPR和接种鹅胚增殖的GPV进行PCR扩增 ,其结果引物GRP1 /GPR2能特异性地扩增GPVVP1 VP3区段核苷酸序列 ,扩增出与设计核苷酸片段大小相符的 0 6kb的序列 ,而对照的鹅副粘病毒cDNA及鸭瘟病毒 (DPR)DNA对照组均出现阴性结果。
According to the gene sequence of GPV H1 strain,a pair of primers were designed by Premier 5 0 and Oligo 4 1 to amplify VP1 VP3 gene from GPV by PCR.The reasult of PCR showed that this pair of primers could specifically amplify VP1 VP3 gene of GPV in infected organs and multiplicated goose embryos.The size of amplified products was 0 6kb. However, the control PCR of cDNA from Goose paramyxovirus and DNA from DPV showed negative.
出处
《畜牧与兽医》
北大核心
2003年第6期8-10,共3页
Animal Husbandry & Veterinary Medicine
基金
黑龙江省"十五"攻关课题 (合同号GB0 1B50 2 0 2 )
