摘要
目的探讨银杏叶提取物对内皮细胞凋亡的影响及其作用机制。方法将孵育好的人脐静脉内皮细胞分成5组:内皮细胞组(对照组);内皮细胞+游离脂肪酸(FFA)组(实验1组);内皮细胞+FFA+10μl银杏叶提取物0.35mg/L组(实验2组);内皮细胞+FFA+10μl银杏叶提取物3.5mg/L组(实验3组);内皮细胞+FFA+10μl银杏叶提取物35mg/L组(实验4组)。分别测定丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX-1)以及细胞凋亡率。结果与对照组比较,实验1组内皮细胞上清液中SOD和GPX-1含量显著降低(P<0.01),而MDA则显著升高(P<0.01);与实验1组比较,实验2、3、4组内皮细胞上清液中SOD和GPX-1含量显著升高(P<0.01),MDA显著降低(P<0.01)。实验1组FFA诱导的内皮细胞凋亡率显著高于对照组(P<0.01);经银杏叶提取物注射后,实验2、3、4组内皮细胞凋亡率显著低于实验1组(P<0.01)。结论银杏叶提取物可通过改善血管内皮细胞的氧化应激水平而抑制其凋亡。
Objective To study the effect of Ginkgo biloba extract on apoptosis of endothelial cells and its mechanism.Methods The incubated human umbilical vein endothelial cells fell into five groups：endothelial cell group（the control group）;endothelial cell＋FFA group（the experimental group 1）;endothelial cell＋FFA＋10μl Ginkgo biloba extract 0.35mg/L group（the experimental group 2）;endothelial cell＋FFA＋10μl Ginkgo biloba extract 3.5mg/L group（the experimental group 3）;endothelial cell＋FFA＋10μl Ginkgo biloba extract 35 mg/L group（the experimental group 4）.Superoxide dismutase（SOD）,glutathione peroxidase（GPX-1）,malondialdehyde（MDA）,and rate of cell apoptosis were respectively measured.Results Compared with the control group,SOD and GPX-1in endothelial cell supernatant of the experimental group 1significantly decreased（P〈 0.01）,MDA significantly increased（P〈 0.01）.Compared with the experimental 1,SOD and GPX-1in endothelial cell supernatant of the experimental group 2,3,4significantly decreased（P〈 0.01）,but MDA increased significantly（P〈 0.01）.FFA-induced endothelial cell apoptosis of the experimental group 1was significantly higher than that of the control group（P〈0.01）;After the injection of Ginkgo biloba extract intervention,endothelial cell apoptosis of the experimental group 2,3,4was significantly lower than that of the experimental group（P〈 0.01）.Conclusion Ginkgo biloba extract can improve the level of oxidative stress in vascular endothelial cells and inhibit the apoptosis.
出处
《临床荟萃》
CAS
2014年第9期1022-1024,共3页
Clinical Focus
基金
河北省重大医学科研项目(zyzd2013020)
河北省科技厅支撑项目(122777126)
关键词
氧化性应激
银杏素
脂肪酸类
非酯化
内皮
血管
oxidative stress
ginkgo biloba extract
fatty acids
nonesterified
endothelium
vascular
