摘要
目的:研究牙周炎对Ⅱ型糖尿病大鼠胰岛细胞凋亡相关蛋白表达的影响。方法:自发Ⅱ型糖尿病OLETF大鼠按照是否复合牙周炎模型随机分为复合组(合并牙周炎)和糖尿病组(牙周健康);同种系同周龄糖耐量正常LETO大鼠随机分为牙周炎组和正常组。复合组和牙周炎组采取丝线结扎联合涂菌建立慢性牙周炎模型,建模20周后处死大鼠,取胰腺组织脱水包埋制作切片。免疫组化半定量分析胰岛组织Bax、Bcl-2和Caspase-3蛋白表达量。结果:正常组和牙周炎组大鼠胰岛细胞Bax、Bcl-2和Caspase-3蛋白表达差异均无显著性(P=0.324,P=0.091,P=0.852);和糖尿病组相比复合组Bax、Caspase-3蛋白表达显著上升(P=0.000,P=0.000),Bcl-2蛋白表达显著下降(P=0.022)。结论:牙周炎对无糖尿病大鼠胰岛细胞凋亡相关蛋白的表达没有影响,但在糖尿病状态下,牙周炎可影响凋亡相关蛋白的表达。
Objective：To evaluate the effects of periodontitis on the expression of apoptosis-related proteins in pancreas of rats with Type 2 Diabetes Mellitus.Methods：Spontaneously type 2 diabetic OLETF rats were randomly divided into 2 groups：diabetes with or without periodontitis（diabetes group and combination group）.LETO rats with the same germline and the same age but having normal glucose tolerance were randomly divided into control group and periodontitis group.20 weeks after periodontitis were established,all the rats were sacrificed and the pancreas were pathologically examined by HE staining.The expression of Bax,Bcl-2 and Caspase-3 in the pancreas islet were detected by immunohistochemistry staining and semi-quantitative analysis.Results：The expression of Bax, Bcl-2 and Caspase-3 in the pancreas islet was no significant difference between control and periodontitis groups（P=0.324,P=0.091,P=0.852）.Compared with diabetes group,the expression of Bax and Caspase-3 in combination group showed a significant increase（P=0.000,P=0.000）,and the expression of Bcl-2 was significantly decreased（P=0.022）.Conclusion：Under healthy conditions,periodontitis has no effect on the expression of apoptosis-related proteins in rat pancreas islet.However,in rats with diabetes,periodontitis may affect the expression of apoptosis-related proteins in pancreas islet.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2014年第4期464-468,共5页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:81371151
81271160)
广东省自然科学基金(编号:S2012010010393)
广东省医学科研基金(广东省卫生厅指令性计划)(编号:C2012033)
关键词
牙周炎
Ⅱ型糖尿病
凋亡相关蛋白
Periodontitis
Type 2 Diabetes Mellitus
Apoptosis- related proteins
