摘要
本试验旨在研究纤维寡糖(COS)和益生菌(PRO)对热应激肉鸡肠屏障功能的影响。将900羽21日龄罗斯(Ross)308肉鸡分为5个组:空白对照(Con)组、热应激(HS)组、益生菌(HS+PRO)组、纤维寡糖(HS+COS)组、合生素(HS+SYN,SYN=PRO+COS)组。Con组鸡舍温度22℃;其余各组鸡舍温度08:00—18:00为33℃,18:00至次日08:00为22℃。饲养试验期为21 d。结果表明:1)与Con组相比,HS组肉鸡平均日增重和平均日采食量分别显著降低10.65%和8.04%(P<0.05);盲肠内容物乳酸杆菌数显著下降(P<0.05),大肠杆菌和梭菌数显著上升(P<0.05);空肠绒毛高度和绒毛高度/隐窝深度显著降低(P<0.05);空肠跨膜电阻(TER)值显著下降(P<0.05),荧光素异硫氰酸酯-葡聚糖(FD4)通透性显著升高(P<0.05)。2)与HS组相比,HS+PRO组和HS+COS组平均日增重、平均日采食量和料重比差异不显著(P>0.05),空肠绒毛高度和绒毛高度/隐窝深度显著升高(P<0.05),FD4通透性显著下降(P<0.05);HS+PRO组盲肠内容物乳酸杆菌、双歧杆菌、大肠杆菌和梭菌数差异不显著(P>0.05);HS+COS组盲肠内容物乳酸杆菌数显著上升(P<0.05),大肠杆菌和梭菌数显著下降(P<0.05)。3)与HS组相比,HS+SYN组肉鸡料重比显著降低4.21%(P<0.05);盲肠内容物乳酸杆菌和双歧杆菌数显著上升(P<0.05),大肠杆菌和梭菌数显著下降(P<0.05);空肠绒毛高度和绒毛高度/隐窝深度显著升高(P<0.05);空肠TER值显著升高(P<0.05),FD4通透性显著下降(P<0.05)。结果提示,热应激使肉鸡生长性能下降,肠屏障功能受损,饲喂益生菌和纤维寡糖可改善上述变化,且两者组合添加效果优于单独添加。
This test was conducted to study the effects of cello-oligosaccharide and probiotics on intestinal bar-rier function of broilers in heat stress. Nine hundred 21-d-old Ross 308 broilers were randomly allocated to 5 groups：control（ Con）group,heat stress（ HS）group,probiotics（ HS＋PRO）group,cello-oligosaccharide （ HS＋COS）group and synbiotics（ HS＋SYN,SYN=PRO＋COS）group. Birds were raised at 22 ℃ in Con group,and the others subjected to cyclic heat stress by exposing them to 33℃ from 08：00 to 18：00 and 22℃from 18：00 to next day 08：00. Raising experiment period was 21 d. The results showed as follows：1）com-pared with birds kept in Con group,average daily gain and average daily feed intake of birds in HS group was significantly reduced（ P〈0. 05 ）;the viable counts of Lactobacillus in cecal contents was significantly de-creased（P〈0.05）,and the viable counts of Escherichia coli and Clostridium in cecal contents were signifi-cantly increased（P〈0.05）;jejunal villus height was significantly shorter（P〈0.05）,and the ratio of villus height to crypt depth was significantly lower（ P〈0.05）;jejunal transepithelial electrical resistance（ TER）val-ue was significantly decreased（P〈0.05）,and the level of jejunal paracellular permeability of 4 kDa-FITC dextran（FD4）flux was significantly raised（P〈0.05）. 2）As compared with HS group,the average daily gain,average daily feed intake and feed to gain ratio of birds in HS＋PRO and HS＋COS groups were not sig-nificantly different（P〉0.05）;villus height and villus height to crypt depth ratio were significantly increased （P〈0.05）;flux of FD4 was significantly reduced（P〈0.05）. As compared with HS group,viable counts of Lactobacillus,Bifidobacterium,Escherichia coli and Clostridium in cecal contents in HS＋PRO group were not significantly different（ P〉0. 05 ）,the viable counts of Lactobacillus in cecal contents was significantly in-creased（ P〈0.05）,and Escherichia coli and
出处
《动物营养学报》
CAS
CSCD
北大核心
2014年第8期2240-2245,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
农业部948(2011-G7)
浙江省重点科技创新团队计划(No.2011R50025)
关键词
纤维寡糖
益生菌
热应激
肉鸡
肠屏障
cello-oligosaccharid
probiotic
heat stress
broiler
intestinal barrier
