摘要
试验旨在研究天冬酰胺(Asn)对脂多糖(LPS)刺激仔猪肠道结构及抗氧化能力的影响。选用24头断奶仔猪,分为对照组、LPS组、0.5%Asn组(0.5%Asn+LPS)和1.0%Asn组(1.0%Asn+LPS)。第24天,各组分别注射LPS或等量生理盐水24 h后屠宰。结果表明:LPS刺激导致空肠绒毛高度显著降低,日粮中添加0.5%或1.0%Asn显著降低了空肠、回肠的隐窝深度,提高了空肠、回肠的绒毛高度和绒毛高度/隐窝深度的比值(P<0.05);LPS刺激也导致空肠超氧化物歧化酶(SOD)活性和氧化型谷胱甘肽(GSSG)/还原性谷胱甘肽(GSH)比值显著升高(P<0.05);日粮中添加0.5%或1.0%Asn显著提高了空肠、回肠GSH的含量(P<0.05),降低了空肠、回肠GSSG/GSH的比值(P<0.01)和丙二醛(MDA)的含量(P<0.05)。结果显示,Asn缓解了LPS刺激引起的肠道结构损伤和抗氧化能力下降。
This experiment was conducted to investigate the effects of asparagine （Asn） on the intestinal morphology andantioxidant capacity of piglets after lipopolysaccharide（LPS） challenge. Twenty-four piglets were allotted to four treatments： control group, LPS group, 0.5% Ash group （0.5% Asn＋LPS）, and 1.0% Asn group （1.0% Asn＋LPS）. On 24, the piglets were injected intraperitoneally with LPS or the same dose of physiological saline. At 24 h following LPS challenge, the pigs were slaughtered. The results showed that LPS pigs had a lower villus height in jejunum （P〈0.05）. Dietary supplementation of 0.5 or 1.0% Asn decreased crypt depth, and increased villus height and villus height/crypt depth ratio in jejunum and ileum （P〈0.05）. LPS pigs also had a higher activity of superoxide dismutase （SOD） and oxidized glutathione （GSSG）/reduced glutathione （GSH） ratio in jejunum （P〈0.05）. Dietary supplementation of 0.5 or 1.0% Asn had a higher GSH （P〈0.05）, and a lower GSSG/GSH ratio （P〈0.10） and malondialdehyde （MDA） level in jejunum and ileum （P〈0.05）. These results suggested that Asn alleviates the intestinal injury and oxidative stress induced by LPS challenge.
出处
《中国畜牧杂志》
CAS
北大核心
2014年第5期60-64,共5页
Chinese Journal of Animal Science
基金
教育部新世纪优秀人才支持计划(NCET-10-0158)
国家自然科学基金(31172222
31372318)
湖北省自然科学基金项目(2010CDA050)
湖北省国际科技合作项目(2011BFA008)
武汉轻工大学研究生教育创新计划(2012cx005)
关键词
天冬酰胺
脂多糖
肠黏膜结构
抗氧化
仔猪
asparagine
lipopolysaccharide
intestinal morphology
antioxidant
piglet
