摘要
目的:研究子宫内膜异位症(EMs)患者在位、异位内膜干细胞分离、培养及鉴定方法。方法:采用胶原酶III法进行体外分离、培养子宫内膜异位症患者在位、异位内膜干细胞,倒置显微镜下观察贴壁细胞的生长情况,并运用免疫荧光和RT-PCR技术检测干细胞相关标记物的表达。结果:成功培养出悬浮生长的子宫内膜干细胞球,CD34、CD117抗原及Oct-4因子在子宫内膜细胞中呈持续的阳性表达。结论:EMs患者在位、异位内膜中存在干细胞,对其分离、培养,可作为研究EMs发病机制的体外模型。
Objective ： To study stem cell isolation, culture and identification of endometriosis （EMs） patients. Methods ： By using collagenase III, stem cells of endometriosis were isolated and cultured in vitro. The growth of cells was observed under inverted microscope and by using immunofluorescence and RT - PCR technique, the expression of stem cells related markers was detected. Results：The suspended growth of endometrial stem cells was successfully developed. CD34, CD117 antigen and Oct -4 factor in endometrial cells continued to have positive expression. Conclusion：In patients with EMs, stem cells exist in the ectopic endometrium. The isolation as well as culture can be used for research in vitro.
出处
《中华中医药学刊》
CAS
2014年第8期1907-1908,I0009,I0010,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81302997)
高等学校博士学科点专项科研基金项目(20113107120004)
上海市教委预算内基金项目(2011JW38)
上海市"杏林新星"培养计划项目(20130037)
