摘要
【目的】建立一种用高效液相色谱法快速检测鸡肉组织中5种氟喹诺酮类药物（诺氟沙星、环丙沙星、达氟沙星、恩诺沙星、沙拉沙星）残留量的方法．【方法】样品的提取液由乙腈与三氯乙酸溶液（质量分数2％）按体积比1∶3的比例混合而成，流动相为乙腈-磷酸/三乙胺溶液（0.02 mol· L-1），荧光激发波长280 nm，发射波长450 nm．【结果和结论】添加低、中、高浓度水平时，回收率为84.71％～99.66％，变异系数为0.20％～4.34％．该方法检测诺氟沙星、环丙沙星和恩诺沙星的残留定量限为10μg · kg-1，达氟沙星的残留定量限为2μg · kg-1，沙拉沙星的残留定量限为20μg · kg-1．此法操作简单、实用，灵敏度高，可以满足鸡肉食品中氟喹诺酮类药物残留检测的需要．
Objective A method for rapid determination of five fluoroquinolones （ norfloxacin, ciprofloxa-cin, danofloxacin, enrofloxacin, sarafloxacin） residues in chicken muscle using high performance liquid chromatography （ HPLC） was established .[Method] The extracting solution of the chicken samples in-cluded a mixture of acetonitrile and trichloroacetic acid （ the mass fraction of 2%） .HPLC mobile phase consisted of acetonitrile and phosphate buffer （0.02 mol· L-1） with triethy lamine.Fluorometric excita-tion wavelength was 280 nm, and emission wavelength was 450 nm.[Result and conclusion] The recov-eries of 5 fluoroquinolones were 84.71%-99.66%at three spiked level（low, medium, high）, and the relative standard deviations （ RSD） were 0.20%-4.34%.The limit of quantiation （ LOQ） of norfloxa-cin, ciprofloxacin and enrofloxacin was 10 μg· kg -1 .The LOQ of danofloxacin was 2 μg· kg -1 , and the sarafloxacin was 20μg· kg -1 .The method is simple , practical , and sensitive .It can meet the needs of determination of fluoroquinolones in chicken muscle .
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2014年第5期14-18,共5页
Journal of South China Agricultural University
基金
省部产学研结合项目(2011B090400228)
关键词
高效液相色谱法
氟喹诺酮类药物
鸡肉
药物残留
方法优化
high performance liquid chromatography
fluoroquinolones
chicken muscle
residue
opti-mization
