摘要
为同时测定水产品中4种氟喹诺酮类药物(环丙沙星、恩诺沙星、诺氟沙星、沙拉沙星)的残留量,对高效液相色谱法进行优化,样品采用酸化乙腈提取,正己烷脱脂。结果:采用流动相2 mL进行洗脱,流动相乙腈∶四丁基溴化铵溶=7∶93,4种氟喹诺酮类药物在0.001~0.1μg/mL线性关系良好,相关系数均>0.999;在10~40μg/kg加标浓度下,回收率为72.40%~83.68%,相对偏差(RSD)为1.30%~6.94%。结果表明:优化后的方法回收率高,重复性和分离效果好,能够用于水产品中氟喹诺酮类药物残留检测。
In order to determine the residual amount of 4 fluoroquinolones residues including ciprofloxacin,enrofloxacin,norfloxacin and ciprofloxacin in aquatic products,the HPLC method was optimized.Samples were extracted with acidified acetonitrile and degreased with n-hexane.The results showed that the mobile phase 2 mL machine-elution,the optimized mobile phase acetonitrile∶tetrabutylammonium bromide dissolved=7∶93 had good linearities in the ranges of 0.001~0.1μg/mL with the correlation coefficients of more than 0.999.The recoveries were 72.40%~83.68%with relative standard deviations in the range of 1.30%~6.94%at 10~40μg/kg spiked concentration.The optimized method has high recovery rate,good repeatability and separation effect,which can be used for the detection of fluoroquinolone residues in aquatic products.
作者
兰承兴
田雪莲
周开拓
李雪林
甘大勇
Lan Chengxing;Tian Xuelian;Zhou Kaituo;Li Xuelin;Gan Dayong(Fuquan City Work Bureau for Rural,Fuquan Guizhou 550500,China)
出处
《贵州畜牧兽医》
2018年第2期27-31,共5页
Guizhou Journal of Animal Husbandry & Veterinary Medicine
关键词
水产品
氟喹诺酮
残留
高效液相色谱
优化
Aquatic Products
Ffluoroquinolones
Residue Detection
High Performance Liquid Chromatography
Optimization
