摘要
运用ISSR分子指纹技术检测甘薯基因组DNA的多态性,是鉴定甘薯遗传多样性和亲缘关系有效的方法之一。本研究选用6个ISSR标记对17份甘薯品种进行了DNA指纹扩增,共扩增出55条谱带,其中多态性谱带50条,多态性水平为90.91%;UBC825引物扩增出带型最多,有16种;根据Nei’s遗传距离计算获得的聚类树状图表明,在GS为0.625时,参试的17份甘薯品种分为4个组群;遗传相似性分析表明,顶芽菜用品种间遗传相似系数最高0.877,叶柄食用品种和烤薯型品种之内的遗传相似系数分别为0.778和0.740。结果表明甘薯主要食用间具有较好的遗传多样性,但同类型品种间却又有较高的遗传相似性,这类品种需要引进或创制新资源。
Scanning the polymorphisms of genome DNA using ISSR molecular fingerprint technology was the most immediate and effective ways to assess the genetic diversity and relationships in sweet potato（ Ipomoea batatas Lam.）germplasm. In this paper,six primers of ISSR molecular marker were used to fingerprint 17 sweet potato accessions. It was shown that 55 bands were amplified,of which 50 bands showed polymorphic among accessions with the polymorphic level of 90. 91%. The primer UBC 825 could amplify 16 band patterns for all accessions,representing the most bands amplified among all primers. Based on Nei＇s distance,the dendrogram showed that all accessions could be divided into four groups at 0. 625 score of genetic similarity（ GS）. Comparison of GS among tested accessions,it was indicated that the score of GS between two groups with top buds for vegetable usage was at the highest level of 0. 877. The GS among petiole-usage group and roasting-usage group were 0. 778 and 0. 740,respectively. It was suggested that the genetic diversity were higher among edible sweetpotato germplasm,whereas in the same usage category,the genetic similarities showed slight high. Therefore it needs to introduce or breed certain novel germplasms.
出处
《核农学报》
CAS
CSCD
北大核心
2014年第7期1197-1202,共6页
Journal of Nuclear Agricultural Sciences
基金
国家现代农业产业技术体系专项经费(nycytx-16-c-20)
浙江省重点项目薯类新品种选育及超高产栽培技术研究(2008C12080)
关键词
甘薯
ISSR标记
分子指纹
遗传多样性
Ipomoea batatas Lam.
ISSR marker
Molecular fingerprint
Genetic diversity
