摘要
以老芒麦×紫芒披碱草种间杂种F1代的幼穗（2～4cm）为外植体材料,以MS为基本培养基,研究了4种不同外源激素组合对幼穗愈伤组织诱导和植株再生能力的影响,筛选出最适杂种F1愈伤组织诱导的培养基为：MS＋2,4-D2.0mg/L,诱导率达63.3％;适宜幼苗分化的最佳培养基为：MS＋6-BA2.0mg/L＋NAA0.4mg/L,分化率为75％;适宜生根的最佳培养基为MS＋IBA0.5mg/L,生根率达90％;组培苗经过炼苗移栽得到了完整植株.该再生体系的建立为下一步进行秋水仙碱诱导染色体加倍提供基础,最终克服其不育性,创造饲草新种质.
In this study, with the young spikes （2-3cm） of hybrid F1 of Elymus scibiricus × E.purpuraristatus as explants and MS as basic medium, the effects of 4 kinds of hormones （2,4-D,6- BA,NAA and IBA） on callus induction, plantlet differentiation and rooting were studied. The optimal medium for callus induction of hybrid F1 was MS＋2,4-D 2.0mg/L, with the induction rate of 63.3%;The optimal medium for plantlets differentiation was MS＋6-BA 2.0mg/L＋NAA 0.4mg/L,with the induction rate of 75%. The optimal medium for rooting was MS＋IBA 0.5 mg/L, with the induction rate of 90%;and the regenerated plant was obtained after acclimatization and transplanting. This regeneration system provides conditions for the following colchicin-induced chromosome doubhng, and ultimately overcoming its sterility to create new grass germplasm resource.
出处
《内蒙古农业科技》
2014年第3期12-14,共3页
Inner Mongolia Agricultural Science and Technology
基金
国家自然科学基金资助项目(31302021)
内蒙古自然科学基金资助项目(2010MS0502)
关键词
老芒麦
紫芒披碱草
种间杂种F1
愈伤组织诱导
植株再生
Elymus sibiricus
E.purpuraristatus
Interspecific hybrid F1
Callus tissue induction
Plant regeneration
