摘要
以绒毛狼尾草幼嫩花穗为材料,研究了培养基种类和2,4-D对愈伤组织诱导以及6-BA对分化的影响。结果表明,MS较N6培养基适合绒毛狼尾草的组织培养。在附加3 mg·L-1 2,4-D的MS培养基中愈伤组织诱导率最高,达到100%。分化培养基以附加1.0 mg·L-1 6-BA的MS培养基为最佳,分化率为44.0%。在1/2MS培养基中附加0.5 mg·L-1 NAA和0.3 mg·L-1活性炭进行生根培养,根系生长健壮,生根率达100%。
The effects of media types and concentration of 2,4-D on callus-induction with young spike of Pennisetum setaceum,and some concentrations of 6-BA on callus differentiation were studied.The result shows MS is the optimal medium on callus-induction.The callus induction rate reached its maximum of 100% at MS media with 3 mg·L-1 2,4-D,in the differentiation media with 1.0 mg·L-1 6-BA,the optimum differentiation rate was achieved at 44.0%,and in the 1/2 MS media plus 0.5 mg·L-1 NAA and 0.3 mg·L-1 activated charcoal,root generation rate reached 100%.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2011年第2期255-258,共4页
Journal of Anhui Agricultural University
基金
北京农业职业学院院级科研基金项目(XY-QN-09-18)资助
关键词
绒毛狼尾草
幼穗
组织培养
植株再生
Pennisetum setaceum
young spike
tissue culture
plant regeneration
