摘要
以三倍体薄皮甜瓜授粉后30d未成熟胚为试材,采用组织培养的胚培养方法,对三倍体薄皮甜瓜芽的再生进行了研究。结果表明:当TDZ浓度为0.04mg/L时,未成熟胚接种第5天出芽率就达到最大值,出芽整齐一致。在M5激素配比组合(6-BA 1.0mg/L,IAA 0.04mg/L)培养基中,平均再生芽数最高,芽的质量最好。在S2(6-BA 0.05mg/L)培养基中,芽伸长量最多,生长状态最好。
Taking immature embryos of the triploid muskmelon which grew 30days after pollination as material,using tissue culture of embryo culture,the regeneration buds of triploid muskmelon were studied.The results showed that in the budding induced immature embryo,the highest seedling rate was obtained on MS medium containing TDZ 0.04mg/L after five days.The highest frequency shoot regeneration was obtained on MS medium containing 6-BA 1.0mg/L,IAA0.04mg/L.The quality of buds was the beat,the adventitious shoots transferred into MS medium containing 6-BA0.05mg/L,the buds elongated more and grew best.
出处
《北方园艺》
CAS
北大核心
2014年第13期106-109,共4页
Northern Horticulture
基金
黑龙江省哈尔滨市青年科技基金资助项目(2012RFQYN043)
关键词
三倍体薄皮甜瓜
组织培养
未成熟胚
不定芽
triploid muskmelon
tissue culture
immature embryo
adventitious bud
