摘要
目的 评价肺癌患者血清和组织中p53蛋白检测的临床意义。方法 采用酶联免疫吸附试验检测 60例术前肺癌患者及 80例对照 (2 0例肺良性病变、60例健康者 )血清中的p53蛋白 ;应用免疫组化方法检测相应 60例肺癌病人的肿瘤组织、正常组织及 2 0例肺良性病变组织中p53蛋白 ,对照分析二者的临床意义。结果 ① 60例肺癌患者中 2例血清p53蛋白阳性 ,对照组均为阴性 ;② 60例肺癌组织中 43例阳性 (72 % ) ,其中包括 2例血清中阳性病人。 2 0例肺良性病变组中 3例阳性 (1 5 % ) ,正常组织中无一例阳性。肿瘤组织中p53蛋白阳性率显著高于对照组 (P <0 .0 1 )。结论 ①p53蛋白检测对肺癌具有诊断价值 ;②血清中p53蛋白阳性率低于肺癌组织中阳性率 ,但其特异性高 ,血清p53蛋白检测可作为肺癌无创性诊断工具 。
Objective To evaluate the clinical significance of p53-protein determination in patients with lung cancer. Methods The p53-protein levels in serum and tumor tissue of 60 patients with lung cancer and 80 controls (20 cases of benign lung diseases and 40 healthy individuals ) was determined by ELISA and immunohistochemistry respectively. Results (1)Only 2 out of the 60 cance patients' serum specimens were positive and the control cases were all negative.(2)The p53-protein positive rates in cancer tissue, benign lung tissue and normal lung tissue were 72%, 15% and 0% respectively. The positive rate in cancer tissue was higher than in the control (P<0.01) and was not related to the clinical stage and histological type. Conclusion (1)The p53-protein determination is of clinical value for the diagnosis of lung cancer.(2)The positive rate of p53-protein is low in sera. As its specificity is 100%, it can serve as an non-traumatic diagnostic tool for lung cancer.
出处
《徐州医学院学报》
CAS
2002年第4期303-305,共3页
Acta Academiae Medicinae Xuzhou
基金
江苏省自然科学基金 (BJ981 1 0 )
江苏省卫生厅重点课题基金 (980 8H)资助
