摘要
目的 探讨Epstein -Barr病毒感染对人类胃肿瘤发生、分化程度及淋巴结转移的影响。方法 取冰冻组织及石蜡切片提取DNA ,用PCR方法扩增EBV -DNABamHI-W片段 ,阳性者提示有EBV感染。分析EBV阳性胃肿瘤的分化程度、淋巴细胞浸润和淋巴结转移情况。结果 1 0 9例胃癌中共有1 8例检出EBV -DNA(阳性率为 1 6.5 % ) ,3例胃间质细胞肿瘤中有 1例EBV -DNA阳性 (阳性率为 33.3% )。EBV阳性胃肿瘤分化程度低 ,淋巴结转移率低 (r =- 0 .1 76,P =0 .0 31 ) ,富于淋巴细胞浸润。结论 EB病毒感染可能参与胃肿瘤的发生。Epstein
Objective: To investigate the influence of Epstien-Barr virus infection on carcinogenesis, differentiation and lyphaden metastasis of gastric neoplasm .Methods: We extracted DNA genomes from frozen tissues and paraffin sections.Polymerase chain reaction(PCR) was used to amplify 129-bp fragment in BamHI-w region of EBV-DNA and the presence of EBV-DNA was evaluated.We analyzed the condition of differentiation, the infiltration of lymphocyte and lymphaden metastasis of EBV-positive gastric neoplasm. Results: EBV was observed in 18(16.5%) of 109 gastric carcinomas and in 1(33.3%) of 3 gastric stromal tumours .The rate of lymphaden metastasis in EBV-positive gastric carcinomas was lower than that in EBV-negative gastric carcinomas( r=-0.176, P=0.031).The EBV-positive gastric carcinomas was liable to be infiltrated by lymphocyte. Conclusions: EBV infection possibly affected the process of gastric carcinogenesis and negatively correlated with lymphaden metastasis of gastric carcinomas.
出处
《南华大学学报（医学版）》
2002年第1期11-13,18,共4页
Journal of Nanhua University(Medical Edition)
关键词
胃肿瘤
腺癌
间质细胞肿瘤
EB病毒
检测
gastric neoplasm
adenocarcinoma
stromal tumours
Epstein-Barr virus(EBV)
