摘要
目的探讨长沙地区汉族吸烟人群中维生素D受体(VDR)基因多态性与慢性阻塞性肺疾病(COPD)易感性的关系。方法针对长沙地区汉族吸烟人群,共收集50例在长沙市第三医院呼吸内科门诊就诊的有吸烟史的COPD稳定期患者,设为吸烟COPD组;同时收集50例在同院同期体检中心体检的吸烟健康者,设为吸烟对照组。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测VDR基因多态位点Apa I、Bsm I以及Taq I的基因型,探讨VDR基因多态性与COPD易感性的关系。结果吸烟COPD组与吸烟对照组之间Apa I位点的aa、Aa、AA基因型频率分布差异有统计学意义(P<0.05)。以Aa+AA基因型做参照,携带aa基因型可能增加吸烟人群COPD的发病风险(OR=2.786,95%CI:1.215~6.389)。2组的a、A等位基因频率分布差异有统计学意义(P<0.05),且与携带等位基因A相比,携带等位基因a的个体可能增加吸烟人群COPD的发病风险(OR=1.897,95% CI:1.042~3.457)。2组Bsm I与Taq I位点的基因型频率分布差异无统计学意义(P>0.05)。结论Apa I的基因多态性可能增加吸烟人群COPD的易感性,而Bsm I以及Taq I位点多态性可能与吸烟人群COPD的易感性无关联。
Objective To detect the correlations between polymorphisms of vitamin D receptor (VDR) genes and chronic obstructive pulmonary disease (COPD) in smokers.Methods Fifty smoking COPD patients and fifty smoking volunteers were enrolled in the study.Polymorphisms of VDR genes including Apa I, Bsm I and Taq I were detected by using polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP). Correlations between VDR gene polymorphisms and the susceptibility to COPD in smokers were analyzed.Results There was a statistically significant difference in the frequency distributions of aa, Aa and AA genotypes between the smoking COPD group and the smoking control group (P<0.05). Using the Aa+ AA genotype as a reference, individuals carrying the aa genotype increased the risk of COPD in smokers (OR=2.786, 95%CI: 1.215-6.389). There was a statistically significant difference in the frequency distributions of a and A alleles between the two groups (P<0.05). Compared with carrying allele A, individuals carrying allele a increased the risk of COPD in smokers (OR=1.897, 95% CI: 1.042-3.457). There was no statistically significant difference in genotype frequency distribution of Bsm I and Taq I between the two groups.Conclusions Apa I gene polymorphism may increase the susceptibility of COPD in smokers, while Bsm I and Taq I gene polymorphisms may not be associated with the susceptibility of COPD in smokers.
作者
张平
朱应群
李喆
范杜
何龙培
Zhang Ping;Zhu Yingqun;Li Zhe;Fan Du;He Longpei(Department of Respiratory Medicine,the Third Hospital of Changsha,Changsha 410015,China)
出处
《国际呼吸杂志》
2019年第2期91-95,共5页
International Journal of Respiration
基金
湖南省卫生计生委科研计划课题项目(B2016165).
关键词
肺疾病
慢性阻塞性
维生素D受体
多态性
吸烟人群
Pulmonary disease, chronic obstructive
Vitamin D receptor
Polymorphism
Smokers
