摘要
目的评价右美托咪定对大鼠内脏痛的影响及蓝斑核α2肾上腺素能受体在其中的作用。方法成年雄性健康SD大鼠32只,体重250~300g,采用随机数字表法分为4组(n=8):对照组(C组)、内脏痛组(VP组)、右美托咪定组(DEX组)和α2肾上腺素能受体拮抗剂阿替美唑组(AP组)。AP组肌肉注射阿替美唑522μg/kg,C组、VP组和DEX组肌肉注射等容量生理盐水;肌肉注射后10min,DEX组和AP组尾静脉注射右美托咪定10μg/kg,C组、VP组尾静脉注射等容量生理盐水;尾静脉注射后15min,VP组、DEX组和AP组腹腔注射0.9%乙酸10ml/kg制备内脏痛模型。于腹腔注射乙酸后60min内记录内脏痛累计评分。于腹腔注射乙酸后2h时,采用免疫组化法检测蓝斑核c-fos阳性细胞数,采用酶联免疫吸附法检测脊髓去甲肾上腺素(NA)含量。结果与C组比较,VP组、DEX组和AP组内脏痛累计评分、蓝斑核c-fos阳性细胞数和脊髓NA含量升高(P<0.05);与VP组比较,DEX组和AP组内脏痛累计评分、蓝斑核c-fos阳性细胞数和脊髓NA含量降低(P<0.05);与DEX组比较,AP组内脏痛评分、蓝斑核c-fos阳性细胞数和脊髓NA含量升高(P<0.05)。结论右美托咪定可减轻大鼠内脏痛,其部分机制与激动蓝斑核α2肾上腺素能受体有关。
Objective To evaluate the effect of dexmedetomidine on visceral pain in rats and the role of α2 adrenergic receptors in locus coeruleus (LC). Methods Thirty-two healthy adult male Sprague-Dawley rats, weighing 250-300 g, were divided into 4 groups (n=8 each) using a random number table method: control group (group C), visceral pain group (group VP), dexmedetomidine group (group DEX) and α2-adrenergic receptor antagonist atipamezole group (group AP). α2-adrenergic receptor antagonist atipamezole 522 μg/kg was intramuscularly injected in group AP, and the equal volume of normal saline was given instead in C, VP and DEX groups.At 10 min after intramuscular injection, dexmedetomidine 10 μg/kg was injected via the tail vein in DEX and AP groups, and the equal volume of normal saline was given instead in C and VP groups.VP, DEX and AP groups received intraperitoneal injection of 0.9% acetic acid 10 ml/kg to make the visceral pain model at 15 min after injection via the tail vein.The cumulative visceral pain score was recorded within 60 min after acetic acid injection.The number of c-fos positive cells in LC was detected by immunohistochemistry, and the content of norepinephrine (NA) in the spinal cord were detected by enzyme-linked immunosorbent assay at 2 h after acetic acid injection. Results Compared with group C, the cumulative visceral pain scores, the number of c-fos positive cells in LC and content of NA in the spinal cord were significantly increased in VP, DEX and AP groups (P<0.05). Compared with group VP, the cumulative visceral pain scores, the number of c-fos positive cells in LC and content of NA in the spinal cord were significantly decreased in DEX and AP groups (P<0.05). Compared with group DEX, the cumulative visceral pain scores, the number of c-fos positive cells in LC and content of NA in the spinal cord were significantly increased in group AP (P<0.05). Conclusion Dexmedetomidine can alleviate visceral pain in rats, and the mechanism is partially related to activating α2 adrenergic receptors
作者
彭晨媚
冷玉芳
张广儒
顾凤香
吕继鹏
Peng Chenmei;Leng Yufang;Zhang Guangru;Gu Fengxiang;Lyu Jipeng(Department of Anesthesiology, First Hospital of Lanzhou University, Lanzhou 730000, China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2018年第10期1227-1229,共3页
Chinese Journal of Anesthesiology
关键词
右美托咪啶
内脏
疼痛
蓝斑
受体
肾上腺素能α2
Dexmedetomidine
Visceral
Pain
Locus coeruleus
Receptors, adrenergic alpha-2
