摘要
目的评价右美托咪定对新生大鼠七氟醚麻醉后远期焦虑状态的影响及中枢不同亚型α2受体在其中的作用。方法清洁健康雄性SD大鼠216只,4~6日龄,体重8~15g,采用随机数字表法分为6组(n=36):对照组(C组)、七氟醚组(S组)、右美托咪定+七氟醚组(D+S组)、右美托咪定+α2受体拮抗剂阿替美唑+七氟醚组(D+A+S组)、右美托咪定+α2A受体拮抗剂BRL44408+七氟醚组(D+B+S组)和右美托咪定+α2C受体拮抗剂JP1302+七氟醚组(D+J+S组)。七氟醚麻醉方法:吸入6%七氟醚麻醉诱导3min,2.1%七氟醚麻醉维持6h。麻醉诱导前30min,D+S组腹腔注射右美托咪定25μg/kg,D+A+S组腹腔注射右美托咪定25μg/kg和阿替美唑250μg/kg,D+B+S组腹腔注射右美托咪定25μg/kg和α2A受体拮抗剂BRL444081.5mg/kg,D+J+S组腹腔注射右美托咪定25μg/kg和α2C受体拮抗剂JP13023mg/kg。麻醉结束后,随机选取12只大鼠处死,收集血样,进行血气分析,并采用ELISA法测定血清皮质酮浓度。剩余大鼠在60日龄时进行高架十字迷宫实验,80日龄时随机选取12只大鼠,进行束缚应激实验。结果与C组比较,S组、D+A+S组和D+B+S组开放臂停留时间百分比降低,总运动距离缩短,麻醉结束后和80日龄束缚应激实验时血清皮质酮浓度升高(P<0.05),D+S组和D+J+S组上述指标差异无统计学意义(P>0.05);与S组比较,D+S组和D+J+S组开放臂停留时间百分比升高,总运动距离延长,麻醉结束后和80日龄束缚应激实验时血清皮质酮浓度降低(P<0.05),D+A+S组和D+B+S组上述指标差异无统计学意义(P>0.05);与D+S组比较,D+A+S组和D+B+S组开放臂停留时间百分比降低,总运动距离缩短,麻醉结束后和80日龄束缚应激实验时血清皮质酮浓度升高(P<0.05),D+J+S组上述指标差异无统计学意义(P>0.05)。结论右美托咪定可减轻新生大鼠七氟醚麻醉后远期焦虑状态,机制可能与激活中枢α2A受体,改善下丘脑-垂体-肾上腺皮质轴功能亢进有关。
Objective To evaluate the effect of dexmedetomidine on the long-term anxiety state after sevoflurane anesthesia in neonatal rats and the role of different central subtypes of α2 receptors. Methods A total of 216 clean-grade healthy male Sprague-Dawley rats, aged 4-6 days, weighing 8-15 g, were divided into 6 groups (n=36 each) using a random number table method: control group (group C), sevoflurane group (group S), dexmedetomidine + sevoflurane group (group D+ S), dexmedetomidine + α2 receptor antagonist atipamezole + sevoflurane group (group D+ A+ S), dexmedetomidine + α2A receptor antagonist BRL44408 + sevoflurane group (group D+ B+ S), and dexmedetomidine + α2C receptor antagonist JP1302 + sevoflurane group (group D+ J+ S). Anesthesia was induced by inhaling 6% sevoflurane for 3 min and maintained by inhaling 2.1% sevoflurane for 6 h. At 30 min before anesthesia induction, dexmedetomidine 25 μg/kg was intraperitoneally injected in group D+ S, dexmedetomidine 25 μg/kg and atipamezole 250 μg/kg were intraperitoneally injected in group D+ A+ S, dexmedetomidine and α2A receptor antagonist BRL44408 1.5 mg/kg were intraperitoneally injected in group D+ B+ S, and dexmedetomidine 25 μg/kg and α2C receptor antagonist JP1302 3 mg/kg were intraperitoneally injected in group D+ J+ S.Twelve rats in each group were randomly selected and sacrificed after the end of anesthesia, blood samples were collected for blood gas analysis, and the serum corticosterone concentration was measured by enzyme-linked immunosorbent assay.The elevated plus maze was performed when the left rats in each group were 60 days old, and 12 rats were selected when the they were 80 days old to perform the restraint stress test. Results Compared with group C, the percentage of time of staying at the open arm was significantly decreased, the total motion distance was shortened, and the serum corticosterone concentration was increased after the end of anesthesia and during the restraint stress test in S, D+ A+ S and D+ B+ S groups (P<0.05), and n
作者
薛金虎
李治松
王中玉
王勇
艾艳秋
杨建军
张卫
Xue Jinhu;Li Zhisong;Wang Zhongyu;Wang Yong;Ai Yanqiu;Yang Jianjun;Zhang Wei(Department of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2018年第10期1214-1218,共5页
Chinese Journal of Anesthesiology
基金
国家自然科学基金(81671094).
关键词
右美托咪啶
焦虑
麻醉药
吸入
婴儿
新生
青少年
受体
肾上腺素能α2
Dexmedetomidine
Anxiety
Anesthetics, inhalation
Infant, newborn
Adolescent
Receptors, adrenergic, alpha-2
