摘要
目的观察丹酚酸B预处理对大鼠心肌缺血/再灌注损伤(MI/RI)能量代谢的作用。方法通过结扎冠状动脉30min再灌注2 h建立大鼠MI/RI模型,随机分为4组:假手术组、模型组及丹酚酸B高、低(20、10 mg/kg)组,于建立模型前7 d开始ip给药,每天1次;再灌注结束后,采用比色法测定血清乳酸脱氢酶(LDH)、肌酸激酶(CK)活力,染色法测定心肌梗死面积(MIA),定磷法测定心肌组织Na+-K+-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶活性。结果与模型组(42.60%)比较,丹酚酸B高、低剂量组的MIA分别缩小至35.93%和37.21%,差异显著(P<0.05);与模型组比较,丹酚酸B高、低剂量组血清CK、LDH活力均显著降低(P<0.05、0.01);与模型组比较,丹酚酸B高、低剂量组心肌组织Na+-K+-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶活性均显著升高(P<0.05、0.01)。结论丹酚酸B预处理可保护MI/RI所致心肌损伤,作用途径可能与改善心肌组织的能量代谢相关。
Objective To investigate the effects of Salvianolic acid B(Sal B)pretreatment against myocardial ischemia/reperfusion injury(MI/RI)in rats.Methods MI/RI rats model was established by 30 min of coronary artery ligation and subsequent 2 h of reperfusion.Rats were randomly divided into four groups(n=10):sham group,model group,and Sal B low(10 mg/kg)and high dose(20 mg/kg)group.Before the modeling operation,Sal B was injected intraperitoneal once daily for 7 d.After the modeling operation,the activities of creatine kinase(CK)and lactate dehydrogenase(LDH)in serum of rats were detected by chromatometry,myocardial infarction area(MIA)in rats were examined by staining method and Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase activities in myocardial tissues were determined by phosphorus method.Results Compared with model group,high and low doses of Sal B significantly reduced MIA to 35.93% and 37.21%,respectively(P<0.05).Compared with model group,the activities of serum CK and LDH activities in Sal B high and low dose groups were significantly decreased(P<0.05,0.01);Compared with model group,the activities of Na^+-K^+-ATPase and Ca^2+-Mg^2+-ATPase in myocardium of Sal B high and low dose groups were significantly increased(P<0.05 and 0.01).Conclusion Sal B pretreatment could protect MI/RI,and its effects maybe related with the improvement of myocardial energy metabolism.
作者
夏杨
张惠军
聂亚莉
XIA Yang;ZHANG Huijun;NIE Yali(Fourth Department of Internal Medicine,Beijing Armed Police Forces Hospital,Beijing 100027,China)
出处
《药物评价研究》
CAS
2018年第12期2210-2213,共4页
Drug Evaluation Research
