摘要
目的建立测定大红袍妇炎宁胶囊中丹参酮ⅡA和丹酚酸B的方法。方法采用RP-HPLC法,Eclipse-XDB-C18(150 mm×4.6 mm,5μm)色谱柱,测定丹参酮ⅡA的流动相为甲醇-水(75:25),检测波长270 nm;测定丹酚酸B的流动相为乙腈-甲醇-1.7%甲酸(10:25:65),检测波长310 nm。结果丹参酮ⅡA3.38~30.42μg.ml-1与峰面积呈线性关系(r=0.9999);丹酚酸B 12.9~116.1μg.ml-1与峰面积呈线性关系(r=0.9998),丹参酮ⅡA的平均回收率为100.9%,RSD=1.31%(n=9);丹酚酸B的平均回收率为100.8%,RSD=1.27%(n=6)。结论所建方法简便、准确、可靠,可用于大红袍妇炎宁胶囊中丹参酮ⅡA和丹酚酸B的含量测定。
OBJECTIVE To establish an HPLC method for the determination of tanshinone ⅡA and salvianolic acid B in Dahongpao Fuyanning capsules.METHODS The HPLC method was carried on Eclipse-XDB-C18(150 mm×4.6 mm,5 μm)column using methanol-water(75:25) and acetonitrile-methanol-1.7% methanonic acid(10:25:65) as the mobile phase,and the detection wavelengths were 270 nm and 310nm.RESULTS The calibration curve for TanshinoneⅡA was linear in the range of 3.38-30.42 μg·ml-1.the calibration curve for Salvianolic acid B was ...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第4期478-480,共3页
West China Journal of Pharmaceutical Sciences
基金
贵州省中药现代化支持项目(黔科合中药专字[2007]5015号)
贵阳市科技局支持项目([2007]筑科农合同字第12-1号)
