摘要
针对磷和微量元素锰对藻类生长的共同作用,研究不同磷源下藻细胞对磷的利用过程与锰作用特性。实验选用铜绿微囊藻为藻种,以硝态氮为氮源,分别以磷酸氢二钾(K_2HPO_4)、小分子有机磷甘油磷酸钠(NaGly)和大分子有机磷卵磷脂(LEC)为磷源,同步考察不同锰(Mn^(2+))浓度下藻类的生长特性。检测指标包括藻密度、溶解性正磷酸盐浓度和碱性磷酸酶活性(Alkaline Phosphatase Activity, APA)。结果表明:以K_2HPO_4为磷源时,藻细胞增长量最大,最大藻密度可达到1.89×107cell/mL,在实验设置的锰浓度(0μg/L<C_(Mn)^(2+)≤100μg/L)范围内,锰浓度的变化未对藻细胞的生长产生影响,藻液中溶解性正磷酸盐含量持续减少,APA持续增加;以NaGly为磷源时,锰浓度对藻细胞生长的影响作用显著,C_(Mn)^(2+)=10μg/L为藻细胞增长的最佳浓度,最大藻细胞密度达到3.86×10~6cell/mL,藻液中溶解性正磷酸盐含量先增加后减少,APA持续增加;以LEC为磷源时,藻细胞基本没有增长,微量元素锰的作用也不显著,藻液中的正磷酸盐含量一直较低,APA相对较高且较稳定。锰元素是叶绿素的重要成分,直接影响藻类的光合作用,不同磷源下微量元素锰对藻细胞的生长状况影响不同,在小分子有机磷源条件下的影响显著。
Aiming at the interaction of phosphorus and trace element manganese on algae growth, the algae utilization process and manganese interaction characteristics under different phosphorus sources were studied under low nitrogen and phosphorus concentration. Microcystis algal species were selected, with nitrate as the nitrogen source, respectively with dipotassium hydrogen phosphate(K2HPO4), small molecule organophosphorus sodium glycerophosphate(NaGly) and macromolecular organic phosphorus lecithin(LEC) as different phosphorus sources, synchronization effects of different manganese(Mn2+)growth characteristics of algae concentration were investigated. The indicators include algal density, dissolved phosphate concentration, and alkaline phosphatase activity(APA). The results show that using K2HPO4 as phosphorus source, algae growth is the largest, with the largest algae density up to 1.89×10^7 cell/mL in manganese concentration experiment(0 μg/L<CMn^2+≤100 μg/L). Within the scope, changes in manganese concentrations did not affect the growth of algae, algae solution dissolved orthophosphate content continued to decrease, and APA increased continuously. With NaGly as phosphorus source, the effect of manganese concentration on algal cell growth was significant, with the optimum concentration of CMn^2+=10 μg/L for algae growth, and the maximum algal cells up to 3.86×10^6 cell/mL. Algae soluble orthophosphate content increased first and then reduced, APA continued to increase. When LEC was used as the phosphorus source, the algae cells did not grow at all,and the effect of trace element manganese was not significant. The content of orthophosphate in the liquid solution was always low, and the APA was relatively high and stable. Manganese is an important component of chlorophyll, which directly affects the photosynthesis of algae. The effects of trace element manganese on the growth of algal cells are different under different phosphorus sources, and the effects of small molecular organic phosphorus sources are signi
作者
王举
陈荣
雷振
沈莹
WANG Ju;CHEN Rong;LEI Zhen;SHEN Ying(School of Environment and Municipal Engineering,Xi'an University of Architecture &Technology,Xi'an710055,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2018年第11期9-14,共6页
Environmental Science & Technology
基金
国家水污染控制与治理科技重大专项子课题(2012ZX07313-001-002)
陕西省青年科技新星项目(2013KJXX-55)
关键词
铜绿微囊藻
微量元素锰
有机磷
无机磷
Microcystis aeruginosa
trace element manganese
organic phosphorus
inorganic phosphorus
