摘要
目的 探讨γ射线照射抑制平滑肌细胞 (SMC)增殖的信号传递途径。方法 培养的动脉平滑肌细胞分别接受吸收剂量为 3 5、7 0、14Gy60 Coγ射线照射后 ,以3 H TdR掺入法测定平滑肌细胞增殖程度 ,放射活性法测定蛋白激酶C(PKC)及丝裂素活化蛋白激酶 (MAPK)活性。结果7 0、14Gy60 Coγ射线能明显抑制平滑肌细胞增殖 ,同时有MAPK、PKC活性明显降低。结论 7 0、14Gy60 Coγ射线抑制血管平滑肌细胞增殖可能是通过降低MAPK、PKC活性途径实现的。
Objective To investigate the signal transduction pathways inhibited by 60 Co γ-radiation in cultured vascular smooth muscle cells (VSMC). Methods The cultured VSMC were irradiated with 60 Co γ-radiation of 3.5,7.0,and 14 Gy respectively.VSMC proliferation was measured by +3H-TdR incorporation,while PKC,MAPK activities were determined by radioactivity assay. Results Proliferation of VSMC was inhibited by 7.0,14 Gy 60 Co γ-irradiation and the activities of PKC,MAPK were decreased significantly. Conclusion Inhibitory effect of 7.0,14 Gy 60 Co γ-irradiation on proliferation of VSMC might be resulted from decrease of the activity of PKC,MAPK.;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2002年第1期25-26,共2页
Chinese Journal of Radiological Medicine and Protection
基金
湖北省科委"九五"攻关基金资助项目 ( 96 2P110 1)
