摘要
目的建立不同胰岛素敏感状态的细胞模型，研究不同状态下蛋白激酶B（PKB）的表达。方法对3T3-L1脂肪细胞采用不同浓度葡萄糖（3．0mmol／L、5．5mmol／L和50．0mmol／L）进行培养，应用胰岛素介导的。H-2脱氧D葡萄糖摄取率，评价低糖组、对照组和高糖组3T3-L1脂肪细胞的胰岛素敏感性，并以RTPCR方法检测葡萄糖刺激前后各组细胞中PKB的表达。结果3T3-L1脂肪细胞^3H-2脱氧-D葡萄糖摄取率依次为，低糖组0．80±0．28，对照组0．53±0．33，高糖组0．32±0．13；在相同条件下，3组细胞间的PKBmRNA表达差异无统计学意义；但在葡萄糖刺激后普遍降低，低糖组、对照组和高糖组分别降低了52％、46％和59％，差异有统计学意义（P〈0．05）。结论体外不同的葡萄糖浓度培养条件，可产生3T3L1脂肪细胞不同的胰岛素敏感状态，而细胞在此条件下的PKB表达并无明显差异。
Objective To establish cell models with different insulin sensitivity status and examine the expression of protein kinase B （PKB/Akt） in the same conditions. Methods 3T3-L1 cells were cultured with different glucose concentrations （3.0, 5.5 and 50.0 mmol/L respectively）. Insulin sensitivity, as presented by insulin-induced glucose transport rate in the cells, was tested by ^3H-2-DG incorporation. PKB expression in cells was determined by RT-PCR. Results The insulin-induced glucose transport rate in 3T3-L1 cells was affected by different concentrations of glucose. The transport rate of ^3H -2-DG in cells cultured in low glucose concentration was increased by 152% and 50% compared with high glucose concentration and control group, respectively. There was no difference in PKB mRNA expression among the three groups under the same conditions, whereas there was an obvious reduction of PKB expression in the three groups after the cells were treated with glucose and insulin. Conclusions 3T3-L1 cells cultured with different concentrations of glucose present the different insulin sensitivity status, but the difference of PKB mRNA expression has not been observed in the same condition.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2008年第8期610-612,共3页
Chinese Journal of Geriatrics
基金
国家自然科学基金（30371573）
