摘要
观察固定化多粘菌素B血液灌流对脓毒症大鼠血液中内毒素、TNF α和血液流变学的作用。将 2 4只Wistar大鼠分为固定化多粘菌素B组(LPS +HP+PMB组 )、空灌流组 (LPS+HP组 )和对照组 (LPS模型组 ) ,给大鼠一次性静注内毒素 (LPS) 0 5mg/kg,LPS+HP+PMB组、LPS +HP组于注入内毒素 4h后抽出血液 ,分离出血浆 ,将血浆经特异亲和剂灌流柱或未偶联亲和剂的空灌流柱进行血液灌流 ,灌流后血浆与血细胞混匀回输动物。测定血浆中LPS、TNF α水平及全血粘度和红细胞比容变化。结果发现 ,经特异性亲和灌流柱灌流 1h ,血浆LPS、TNF α水平明显下降 ,提示固定化多粘菌素B血液灌流能显著清除血浆中内毒素 ,改善血液流变学。
To observe the changes in hemorheology, endotoxin and TNF α in blood after hemoperfusion(HP) with adsorbent immobilized polymyxin B (PMB) on sepsis in rats. Wistar rats were randomly divided into three groups:LPS+HP+PMB,LPS+HP and LPS. All the rats received intravenously injection of lipopolysaccharide (LPS, Escherichia coil O111:B4,1mg/kg). Plasma of the rats in the group of LPS+HP+PMB was passed through a column containing sepharose coated activated charcoal immobilized polymyxin B at the 4th hour after LPS injection. The treated plasma was transfused bak after being mixed with blood cells. In LPS+HP group, the column did not contain immobilized polymyxin B. The animals of LPS group received LPS only. Quantitative endotoxin determination in blood was done with limulus amebocyte lysate test,TNF α of the plasma assayed with ELISA, and hemorheology parameters were also observed after hemoperfusion. In LPS+HP+PMB group, the concentration of plasma was significantly decreased after hemoperfusion, but it was still significantly higher than the baseline value, and there was a decrease of blood cell ratio after hemoperfusion. The results suggest that specific adsorbent could remove endotoxin in the circulation and improve hemorheology.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第3期212-213,共2页
Medical Journal of Chinese People's Liberation Army
基金
国家重点基础研究发展规划项目 (编号G1 99990 542 0 3)
重庆市攻关项目 (编号 2 0 0 1 661 3)
