摘要
以半定量RT -PCR技术检测经人类巨细胞病毒 (HCMV)感染和 /或全反式维甲酸 (ATRA)处理的神经胶质瘤细胞U2 5 1株的HOXB5 ,HOXB6 ,HOXB7和HOXB8的相对表达水平 ,以探讨HCMV致畸的可能机制。结果示 ,未经处理的U2 5 1细胞不表达HOXB5 ,HOXB6及HOXB8,但表达HOXB7;HCMV感染和 /或ATRA处理后 ,细胞仍不表达HOXB5及HOXB6 ,但在第二代时均表达HOXB8,尤以HCMV和ATRA共同处理后更明显 ;第三代后HOXB8的表达恢复正常。HCMV和 /或ATRA处理细胞后 ,HOXB7的表达在第一代即上调 ,第二代、第三代其表达稍下降 ,至第四代时表达又上调。表明HCMV致畸作用可能是通过诱导HOX基因的表达进而调节其它基因的异常表达而实现。
Objective The expressions of HOXB5,HOXB6,HOXB7,and HOXB8 genes of U251 cell infected by human cytomegalovirus and/or treated with all trans-retinoic acid(ATRA) were detected by semi-quantitative RT-PCR.The results were that U251 cell did not express HOXB5,HOXB6, and HOXB8 but expressed HOXB7 without infecting HCMV and/or being treated with ATRA.After infected with HCMV and/or treated with ATRA,the expressions of HOXB7 and HOXB8 were up-regulated and the expression of HOXB7 lasted the fourth generation,while HOXB8 was up-regulated only in the second generation.These results indicate that the abnormal expression of HOXB gene induced by HCMV may play an important role in the maldevelopment.
出处
《湖南医科大学学报》
CSCD
北大核心
2001年第5期409-411,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金资助 ( 3970 0 0 0 6 )
