摘要
目的 :探讨应用胶原膜构建组织工程心脏瓣膜的可行性。方法 :扫描电镜观察材料结构特点。材料兔皮下包埋实验 ,分别于 4,6 ,8,12周观察材料的生物相容性和降解率。培养犬主动脉瓣间质细胞、主动脉壁间质细胞和皮肤成纤维细胞 ,对照其生长曲线、平滑肌α肌动蛋白表达和扫描电镜特点。将犬主动脉壁间质细胞和内皮细胞种植于胶原膜上 ,观察其形态并测定细胞合成前列环素的功能。结果 :胶原膜呈网孔状结构 ,孔径 10 7μm。皮下包埋实验显示材料生物相容性好 ,体内降解时间为 12周。犬主动脉瓣间质细胞和主动脉壁间质细胞平滑肌α肌动蛋白均为部分阳性表达 ,细胞内有大量粗面内质网 ,生长曲线相似。种植实验示细胞在材料表面生长良好 ,并具有合成、分泌前列环素 (84.2 pg/ m L vs0 .4pg/ m L ,P<0 .0 5 )的功能。结论 :以胶原膜为支架体外构建组织工程心脏瓣膜细胞不仅能在材料表面生长 ,还能合成、分泌血管活性物质 ,是具有“生理功能 "的组织工程心脏瓣膜。
AIM:To evaluate the feasibility of tissue engineered heart valve on collagen membrane. METHODS: Constructive characteristics of collagen membrane were observed by scanning electron microscope. Collagen membrane was implanted subcutaneously in rabbit and extirpated at 4,6,8,10 weeks in order to study its biocompatibility and biodegradibility. Cell growth curve, smooth muscle(SM) α-actin and ultra-microscopic characteristics were compared between canine aortic valve interstitial cells and cells of aortic wall. Cells of aortic wall and aortic endothelium were seeded on the collagen membrane and their function of PGI_2 synthesis was determined. RESULTS: Porous diameter of collagen membrane was 107 μm. Collagen membrane had good biocompatibility and was completely degraded at the end of the 12th week. Both part of interstitial cells from aortic valve and aortic wall expressed SM α-actin, and their growth curves were similar. Cells implanted on collagen membrane grew well and they could synthesize, secret PGI_2(84.2 pg/mL vs 0.4pg/mL,P<0.05). CONCLUSION: It is feasible to construct a tissue engineered heart valve in vitro on collagen membrane.
出处
《心脏杂志》
CAS
2001年第5期372-376,共5页
Chinese Heart Journal
基金
国家自然科学基金资助项目 ( 39970 733)
