摘要
目的 探讨应用聚乳酸聚乙醇酸 (PL GA)构建组织工程心脏瓣膜的可行性。 方法 扫描电子显微镜观察 PL GA结构特点 ,将 PL GA在兔皮下包埋 ,分别于 2周、4周、6周、8周和 12周观察材料的生物相容性和降解率 ,培养犬主动脉瓣间质细胞、主动脉壁间质细胞和皮肤成纤维细胞 ,对照其生长曲线、平滑肌 α肌动蛋白表达和扫描电子显微镜特点。将犬主动脉壁间质细胞和内皮细胞种植于 PL GA上 ,观察其形态并测定细胞合成胶原和前列环素的功能。 结果 PL GA呈网孔状结构 ,孔径 179μm。皮下包埋显示 PL GA生物相容性好 ,体内降解时间为 12周。犬主动脉瓣间质细胞和主动脉壁间质细胞平滑肌 α肌动蛋白均为部分阳性表达 ,细胞内有大量粗面内质网 ,生长曲线相似。细胞种植显示细胞在材料表面生长良好 ,并具有合成胶原和前列环素的功能 (P<0 .0 5 )。 结论 以 PL GA为支架体外构建组织工程心脏瓣膜细胞不仅能在 PL GA表面生长 ,还能合成细胞间质和血管活性物质 。
Objective To evaluate the feasibility to construct tissue engineered heart valve leaflet with poly lactic acid/glycolic acid(PLGA) scaffold. Methods To observe constructive characteristics of PLGA by scanning electron microscope. PLGA was implanted subcutaneously in rabbit and extirpated at 2, 4,6,8,12 weeks in order to study its biocompatibility and biodegradibility. Cell growth curve, smooth muscle α actin and ultra microscopic characteristics were compared between canine aortic valve interstitial cells and cells of aortic wall. Cells of aortic wall and aortic endothelium were seeded on the PLGA and their function of collagen, prostaglandin I 2 (PGI 2)synthesis were determined. Results Porous diameter of PLGA was 179μm. PLGA had good biocompatibility and was completely degraded at the end of the 12th week. Both part of interstitial cells from aortic valve and aortic wall expressed smooth muscle α actin, and their growth curves were similar. Cells implanted on PLGA grew well and they can synthesize, secret collagen and PGI 2( P <0.05). Conclusion It is feasible to construct an tissue engineered heart valve leaflet in vitro with PLGA scaffold.
出处
《中国胸心血管外科临床杂志》
CAS
2001年第4期235-239,共5页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
国家自然科学基金资助项目 ( 39970 733)
关键词
聚乳酸聚乙醇酸
组织工程
心脏瓣膜
生物材料
Poly lactic acid/glycolic acid
Tissue engineered
Heart valve
Biomaterial
