摘要
目的研究利用猪小肠黏膜下层(small intestinal submucosa,SIS)作为组织工程材料修复SD大鼠背侧皮肤全层缺损的可行性。方法选用6周龄SD大鼠28只,体重300～350g。造成背部上下2处直径2cm的全层皮肤缺损,上处为空白组,不用任何覆盖;下处为SIS组,将解冻的SIS剪成直径2cm的圆形,覆盖缝合缺损皮肤周围。术后3d,1、2、3、4、6和8周(n=4),分别观察缺损皮肤修复的情况,HE染色观察,并计算修复面积百分比。结果两组缺损皮肤大体观察修复时均未出现感染;于2、3、4和6周时SIS修复皮肤的速度快于空白组,修复后的皮肤质软、有弹性,与周围组织基本持平,且瘢痕组织较空白组薄。1、2、3、4、6和8周时皮肤修复面积:SIS组分别为15.72%±3.64%,43.81%±4.87%,65.35%±5.63%,87.95%±4.78%,96.90%±6.89%和100%;空白组分别为13.42%±3.77%,38.24%±5.63%,58.74%±4.48%,76.50%±5.23%,92.30%±5.75%及100%,SIS组于1、2、3、4周时其皮肤修复面积百分比与空白组比较有统计学意义(P<0.05)。组织学观察SIS组,皮肤中形成较多角化细胞及胶原组织,与正常皮肤的组织结构相近;空白组,皮肤中存在较多肉芽组织,皮下胶原中有炎性细胞及含铁血黄素;两组均无毛囊及附件产生。结论SIS作为组织工程支架材料修复大鼠皮肤全层缺损有一定的可行性。
Objective To investigate the feasibility of using the porcine small intestinal submucosa （SIS） as a kind of the new tissue engineered materials to repair the rat full skin defect. Methods Twenty-eight 6-week-old SD rats weighing 300-350 g were selected in this experimental study. Two 2-cm-diameter round full skin defects were made on the rat back. The upper round defect was used as the blank group, which had no coverings, and the lower round defect was used as the SIS group. SIS that had been produced earlier was transplanted in the defected area. At 3 days, 1, 2, 3, 4, 6 and 8 weeks after the transplantation, the observation was made on the repaired skin conditions, the HE stain, and the repaired skin proportion. Results There was no infection in the two groups. The repairing speed in the SIS group was faster than that in the blank group at 2, 3, 4 and 6 weeks after the transplantation. The skin repaired by SIS was soft and elastic in texture, which had the same high level as the normal skin. The scar tissues in the SIS group were thinner than those in the blank group. The repaired skin proportions at 1, 2, 3, 4, 6 and 8 weeks after the transplantation were 15.72%±3. 64%, 43. 81%±4.87%, 65.35%±5.63%, 87.95%±4.78%,96.90%±6.89% and 100%, respectively in the SIS group, and 13.42%±5.63%,58.74%±4.48%,76. 50%±5.23%,92.30%±5.75% and 100%, respectively in the blank group. There was a statistically significant difference between the two groups at 1, 2, 3 and 4 weeks after the transplantation（P〈0. 05）. Under the microscope, the SIS-repaired skin was observed to have more keratinocytes and collagen tissues, which was familiar to the normal skin. Conclusion Porcine SIS can be used as a new kind of the tissue engineered materials to repair the full skin defect.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2007年第8期872-876,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
上海市卫生局青年科研基金资助项目(054Y07)~~
关键词
小肠黏膜下层
皮肤缺损
修复
猪
Small intestinal submucosa Skin defect Repair Porcine
