摘要
目的初步观察激光微孔结构对猪脱细胞真皮基质(ADM)血管化的影响。方法健康雄性裸鼠36只,随机分为实验组及对照组,实验组移植激光微孔猪脱细胞真皮基质(LPADM)加自体薄皮片,对照组移植无孔猪脱细胞真皮基质加自体薄皮片,手术分"二步法"完成。术后1d、3d、14d每组各取6只裸鼠处死,切取标本行组织学及电镜检查。结果实验组术后第1天,扫描电镜发现新生组织通过微孔结构长入ADM内部。组织学观察显示新生组织中有血管样管腔结构,其内有红细胞征象。术后第3天实验组LPADM中新生组织明显长入微孔结构内部。术后14d,LPADM已完成血管化,微孔周围的胶原网架中可见新生的血管形成,微孔结构中的新生组织逐渐转变为胶原纤维为主的真皮组织。对照组在整个实验过程中仅见少量成纤维细胞迁入,未见血管或血管内皮细胞等组织长入。结论微孔结构的存在,可以为创面基底的新生组织向ADM内部的生长提供一条通道,提高ADM的血管化能力。
Objective To observe the effect of laser micropore structure on vascularization of porcine acellular dermal matrix.Methods 36 healthy male nude mice were randomly divided into experimental group and control group.The experimental group were transplanted laser micropore porcine acellular dermal matrix(LPADM)and autologous thin skin,the control group were transplanted non-porous acellular dermal matrix and autologous thin skin,the surgery were completed by two-step.Each group select six nude mice to cut specimen for histology and electron microscopy at 1 d,3 d,14 d after operation.Results Scanning electron microscopy revealed new tissue ingrowth into LPADM by laser micropore structure of the experimental group at 1 d after operation,histological observation showed that there were vessel-like lumen structure in the new organization,and the new organization was obvious ingrowth at 3 d after operation.At 14 d after operation,the LPADM completed revascularization,the new organization in the laser microporous gradually changed into the collagen fiber-based dermal tissue.During the whole experiment,the was no blood vessels or vascular endothelial cells move in the control group.Conclusions The presence of laser microporous structure can improve the vascularization capacity of LPADM,provide a channel of the cells and organization ingrowth.
出处
《中华临床医师杂志（电子版）》
CAS
2012年第18期35-37,共3页
Chinese Journal of Clinicians(Electronic Edition)
基金
浙江省自然科学基金重点项目(Z2080985)
温州市科技计划项目(Y20080101)
鹿城区科技计划资助项目(S070109)
关键词
激光
新生血管化
病理性
微孔结构
脱细胞真皮基质
Lasers
Neovascularization,pathologic
Microporous structure
Acellular dermal matrix
